AB15246

Anti-alpha Tubulin antibody - Loading Control

Name: Anti-alpha Tubulin antibody - Loading Control (ab15246) | Abcam
Brand: Abcam Limited
SKU: AB15246
Price: 400 USD
Availability: InStock
Rating: 5 (18 reviews)

Lab Essentials
What is this?

(18 Reviews)

(309 Publications)

Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) is a rabbit polyclonal antibody detecting alpha Tubulin in Western Blot, IHC-P. Suitable for Human.

- Over 270 publications
- Trusted since 2004

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

2 Images

Unknown

Western blot - Anti-alpha Tubulin antibody - Loading Control (AB15246)

Lanes : 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

Lanes : 1-3 : 4^oC (1 freeze/thaw); 4-6 : 4^oC (2 freeze/thaws); 7-9 : 4^oC (4 freeze/thaws)

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (ab15246)

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-alpha Tubulin antibody - Loading Control (AB15246)

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (ab15246) at 1 µg/mL

Lane 1:

SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

Lane 2:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Homology to both Tubulin alpha-3E chain and Tubulin alpha-4A chain

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gerbil": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Newt": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of alpha Tubulin?
Anti-alpha Tubulin - Microtubule Marker (ab15246) specifically detects a band for alpha Tubulin (UniProt: Q6PEY2) at a molecular weight of 50kDa.

Trusted by the scientific community
Anti-alpha Tubulin - Microtubule Marker (ab15246) was first used in a scientific publication in 2004 and has been cited over 270 times in peer-reviewed journals.

Reviewed by scientists
Anti-alpha Tubulin - Microtubule Marker (ab15246) has over 15 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

style

left-column

Publications (309)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1673427 PubMed41031165

2025

Breaking the cancer code: a novel DNA minicircle to disable STAT3 in ovarian cancer cells SKOV3.

Applications

Unspecified application

Species

Unspecified reactive species

Adina-Gabriela Vasilescu,Andrei-Mihai Vasilescu,Livia Elena Sima,Natalia Baran,Ștefan-Eugen Szedlacsek

Frontiers in pharmacology 16:1684226 PubMed41019995

2025

A novel cytotoxic anti-B7-H3 affibody with therapeutic potential in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Andrei-Mihai Vasilescu,Adina-Gabriela Vasilescu,Livia Elena Sima,Cristian V A Munteanu,Natalia Baran,Ștefan-Eugen Szedlacsek

Science advances 11:eadv2902 PubMed40749059

2025

Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yin,Hsueh-Ling Chen,Anna Grigsby-Brown,Yi He,Myriam L Cotten,Jacob Short,Aidan Dermady,Jingce Lei,Mary Gibbs,Ethan S Cheng,Dean Zhang,Caixia Long,Jennifer Tabet,Lele Xu,Tiffany Zhong,Rinat Abzalimov,Mariam Haider,Rong Sun,Ye He,Qiangjun Zhou,Nico Tjandra,Quan Yuan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70893 PubMed40742315

2025

Increased Dipeptidyl Peptidase-4 Promotes Adipose Inflammation and Dysfunction in Mice Under Chronic Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Meiping Zhang,Huazhen Wang,Xiangdan Li,Shangzhi Shu,Jinshun Piao,Xueling Yue,Songzhen Zhao,Miao Li,Xianglan Jin,Yongshan Nan,Xian Wu Cheng

eLife 13: PubMed40272400

2025

RAS-p110α signalling in macrophages is required for effective inflammatory response and resolution of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Rosell,Agata Adelajda Krygowska,Marta Alcón Pérez,Cristina Cuesta,Mathieu-Benoit Voisin,Juan de Paz,Héctor Sanz-Fraile,Vinothini Rajeeve,Ana Carreras-González,Alberto Berral-González,Ottilie Swinyard,Enrique Gabandé-Rodríguez,Julian Downward,Jordi Alcaraz,Juan Anguita,Carmen García-Macías,Javier De Las Rivas,Pedro R Cutillas,Esther Castellano Sanchez

Aging cell 24:e70023 PubMed40248988

2025

Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Manfrevola,Nicola Mosca,Vincenza Grazia Mele,Teresa Chioccarelli,Guillaume Martinez,Charles Coutton,Monica Mattia,Mariaceleste Pezzullo,Silvia Fasano,Gilda Cobellis,Nicoletta Potenza,Rosanna Chianese

Cancer reports (Hoboken, N.J.) 8:e70096 PubMed39948708

2025

Insulin-Like Growth Factor 2 mRNA-Binding Protein 2 (IGF2BP2) Promotes Castration-Resistant Prostate Cancer Progression by Regulating AR-V7 mRNA Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Taruna Saini,Devesh Srivastava,Rajnikant Raut,Parul Mishra,Ashish Misra

Frontiers in cell and developmental biology 12:1472340 PubMed39839673

2025

Involvement of RBP-J interacting and tubulin-associated protein in the distribution of protein regulator of cytokinesis 1 in mitotic spindles.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Caspers,Andreas Ritter,Badi Bahrami,Samira Catharina Hoock,Susanne Roth,Alexandra Friemel,Franz Oswald,Frank Louwen,Nina-Naomi Kreis,Juping Yuan

Nature communications 16:469 PubMed39774941

2025

Mitotic block and epigenetic repression underlie neurodevelopmental defects and neurobehavioral deficits in congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

George C Gabriel,Hisato Yagi,Tuantuan Tan,Abha Bais,Benjamin J Glennon,Margaret C Stapleton,Lihua Huang,William T Reynolds,Marla G Shaffer,Madhavi Ganapathiraju,Dennis Simon,Ashok Panigrahy,Yijen L Wu,Cecilia W Lo

Molecular cell 84:2900-2917.e10 PubMed39032490

2024

Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Han Lin,Madeline K Jensen,Nathan D Elrod,Hsu-Feng Chu,MaryClaire Haseley,Alissa C Beam,Kai-Lieh Huang,Wesley Chiang,William K Russell,Kelsey Williams,Christoph Pröschel,Eric J Wagner,Liang Tong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Tubulin antibody - Loading Control

Western blot - Anti-alpha Tubulin antibody - Loading Control (AB15246)

Western blot - Anti-alpha Tubulin antibody - Loading Control (AB15246)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (309)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise