Rabbit Polyclonal Alpha-tubulin 4 antibody. Suitable for WB, ICC/IF and reacts with Chinese hamster, Chicken, Cow, Rat, Mouse, Human samples. Cited in 431 publications.
Images
Publications
- International journal of molecular sciences 24:2023Dose-Dependent Effect of Mitochondrial Superoxide Dismutase Gene Overexpression on Radioresistance of HEK293T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina M Tavleeva,Elena E Rasova,Anna V Rybak,Elena S Belykh,Elizaveta A Fefilova,Elizaveta M Pnachina,Ilya O VelegzhaninovPubMed 38139144
- Translational psychiatry 13:3572023Electroacupuncture modulates glutamate neurotransmission to alleviate PTSD-like behaviors in a PTSD animal model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMudan Cai,Hee Ra Park,Eun Jin YangPubMed 37993441
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Chicken | Tested | Expected |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Tested | Expected |
| Cow | Tested | Expected |
| Monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
Species Chicken | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
Species Cow | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
Species Rat | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
Species Mouse | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
Species Human | Dilution info 1 µg/mL | Notes Can be blocked with alpha Tubulin peptide (ab23537). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Xenopus laevis, Chimpanzee, Monkey, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Chicken, Cow, Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Xenopus laevis, Chimpanzee, Monkey, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Alternative names
Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
Recommended products
Rabbit Polyclonal Alpha-tubulin 4 antibody. Suitable for WB, ICC/IF and reacts with Chinese hamster, Chicken, Cow, Rat, Mouse, Human samples. Cited in 431 publications.
Alternative names
Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody detects a single band in cell lines, corresponding to alpha tubulin.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody - Loading Control (ab4074)
ab4074 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4064 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg
Lane 2: NIH/3T3 (Mouse) Whole Cell Lysate (ab52956) at 10 µg
Lane 3: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Image from PLoS One. 2014; 9(10): e109590. Fig 5o,doi: 10.1371/journal.pone.0109590 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody - Loading Control (ab4074)
Immunocytochemistry of SisNPFR-C6E8 cell line. Cells were prefixed in freshly prepared 4% paraformaldehyde (PFA) in F12 K culture medium with 10% fetal bovine serum and Geneticin. Fixed cells were permeabilized in PBST (0.25% Triton X-100 in PBS) for 5 min and blocked at 4°C overnight in 5% normal goat serum. The cells were then incubated at 4°C overnight with ab4074 (1:50). Cover slips were placed onto slides and mounted with VectaShield containing DAPI.
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/mL
Lanes 1 and 5: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
Lanes 2 and 6: HEK-293 (Human) Whole Cell Lysate (ab52256) at 20 µg
Lanes 3 and 7: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 20 µg
Lanes 4 and 8: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/mL
Lane 1: Mouse 3T3 lysate at 20 µg
Lane 2: Rat liver lysate at 20 µg
Lane 3: Cow cell lysate at 20 µg
Lane 4: Chicken cell lysate at 20 µg
Lane 5: CHO lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30s
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