Rabbit Monoclonal Alpha-tubulin 4 tri methyl K40 antibody. Suitable for I-ELISA, Dot, ICC/IF and reacts with Synthetic peptide - Human, Human, Mouse samples.
Images
![Indirect ELISA - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--indirect-elisa-img438921.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--immunocytochemistry-immunofluorescence-img438892.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--immunocytochemistry-immunofluorescence-img438893.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330)")
![Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--dot-blot-img438917.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (AB208330)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| I-ELISA | Dot | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Expected | Tested |
| Synthetic peptide - Human | Tested | Tested | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 62.5 ng/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain
Rabbit Monoclonal Alpha-tubulin 4 tri methyl K40 antibody. Suitable for I-ELISA, Dot, ICC/IF and reacts with Synthetic peptide - Human, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 18-6
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody shows weakly cross-reactivity with the di-methyl peptide.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
- Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
- Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
- Associated diseases and disorders
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Indirect ELISA - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--indirect-elisa-img438921.jpg "Indirect ELISA - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)")
Indirect ELISA - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)
Indirect ELISA analysis of ab208330 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human tubulin unmodified peptide,Human tubulin (mono methyl K40) peptide,Human tubulin (di methyl K40) peptide,Human tubulin (tri methyl K40) peptide,Human tubulin (tri acetyl K40) peptide.
Antigen concentration: 10ng/ml
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--immunocytochemistry-immunofluorescence-img438892.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 786-O (human kidney epithelial cell) cells labelling alpha Tubulin (tri methyl K40) with ab208330 at 1/500 (1.862 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 786-O cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--immunocytochemistry-immunofluorescence-img438893.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling alpha Tubulin (tri methyl K40) with ab208330 at 1/500 (1.862 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330), expandable thumbnail](https://content.abcam.com/products/images/alpha-tubulin-tri-methyl-k40-antibody-18-6-ab208330--dot-blot-img438917.jpg "Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)")
Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330)
Dot blot analysis of alpha Tubulin (tri methyl K40) using ab208330 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: Human tubulin unmodified peptide
Lane2: Human tubulin (mono methyl K40) peptide
Lane3: Human tubulin (di methyl K40) peptide
Lane4: Human tubulin (tri methyl K40) peptide
Lane5: Human tubulin (tri acetyl K40) peptideBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody shows weakly cross-reactivity with the di-methyl peptide.
All lanes: Dot Blot - Anti-alpha Tubulin (tri methyl K40) antibody [18-6] (ab208330) at 1/1000 dilution
Lane 1: Human tubulin unmodified peptide (aa30-50)
Lane 2: Human tubulin (mono methyl K40) peptide (aa30-50)
Lane 3: Human tubulin (di methyl K40) peptide (aa30-50)
Lane 4: Human tubulin (tri methyl K40) peptide (aa30-50)
Lane 5: Human tubulin (tri acetyl K40) peptide (aa30-50)
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Downloads
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