Rabbit Recombinant Monoclonal AP-2 complex subunit alpha-1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (AB249038), expandable thumbnail](https://content.abcam.com/products/images/alpha1-adaptin-antibody-epr7572-bsa-and-azide-free-ab249038--western-blot-img137461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (AB249038)")
![Flow Cytometry (Intracellular) - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (AB249038), expandable thumbnail](https://content.abcam.com/products/images/alpha1-adaptin-antibody-epr7572-bsa-and-azide-free-ab249038--flow-cytometry-intracellular-img50888.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (AB249038)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).
Alternative names
ADTAA, CLAPA1, AP2A1, AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Recommended products
Rabbit Recombinant Monoclonal AP-2 complex subunit alpha-1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7572
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249038 is the carrier-free version of Anti-Alpha1-adaptin antibody [EPR7572] ab151720.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha1-adaptin also known as ADTAA or AP2A1 is an essential component of the adaptor protein complex 2 (AP-2). It has a mass of approximately 108 kDa. This protein is primarily expressed in the cytoplasm of eukaryotic cells facilitating the assembly of clathrin-coated vesicles. Alpha1-adaptin interacts with clathrin and cargo receptor proteins acting mechanically to mediate vesicle formation and endocytosis. Its role spans across various cell types due to its involvement in cellular uptake processes.
- Biological function summary
Alpha1-adaptin plays an important role in regulating endocytosis by assisting the AP-2 complex in selecting and concentrating specific cargo molecules for inclusion in vesicles. This complex is essential for internalizing membrane proteins and lipids at the plasma membrane. The AP-2 complex where Alpha1-adaptin acts as a component interacts with other proteins like clathrin and various membrane receptors ensuring proper cellular distribution of nutrients and signaling molecules.
- Pathways
Alpha1-adaptin is critical in endocytic and trafficking pathways. It associates with proteins such as clathrin and AP-2 complex components which are involved in mediating the endocytic pathway. Alpha1-adaptin assumes a critical role in receptor-mediated endocytosis working alongside other adaptin families. Additionally it is involved in actin cytoskeleton dynamics influencing intracellular transport and cellular signaling pathways.
- Associated diseases and disorders
Alpha1-adaptin's function links to conditions like neurodegenerative diseases and cancer. It interacts with proteins involved in pathological processes such as the epidermal growth factor receptor (EGFR) in cancer which undergoes deregulation and faulty endocytosis. Furthermore defects in alpha-adaptin function may contribute to neurodevelopmental disorders due to impaired synaptic vesicle recycling and neurotransmitter regulation. The AP-2 complex including Alpha1-adaptin is therefore a significant player in understanding the molecular mechanisms of these disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038), expandable thumbnail](https://content.abcam.com/products/images/alpha1-adaptin-antibody-epr7572-bsa-and-azide-free-ab249038--western-blot-img137461.jpg "Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038)")
Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038)
This data was developed using Anti-Alpha1-adaptin antibody [EPR7572] ab151720, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 108 kDa
![Flow Cytometry (Intracellular) - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038), expandable thumbnail](https://content.abcam.com/products/images/alpha1-adaptin-antibody-epr7572-bsa-and-azide-free-ab249038--flow-cytometry-intracellular-img50888.jpg "Flow Cytometry (Intracellular) - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038)")
Flow Cytometry (Intracellular) - Anti-Alpha1-adaptin antibody [EPR7572] - BSA and Azide free (ab249038)
This data was developed using Anti-Alpha1-adaptin antibody [EPR7572] ab151720, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling alpha Adaptin with Anti-Alpha1-adaptin antibody [EPR7572] ab151720 at 1/100 diluton (red), compared to a nonspecific control antibody (green).
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