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AB236626

Anti-ALPK1 antibody [EPR20911-169]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal ALPK1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA1527, LAK, ALPK1, Alpha-protein kinase 1, Chromosome 4 kinase, Lymphocyte alpha-protein kinase

2 Images
Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)
  • WB

Collaborator

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.

Exposure time : 5 minutes

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution

Lane 1:

ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

Wild-type 293T whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)
  • WB

Lab

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

Blocking and Diluting buffer : 5% NFDM /TBST.

To get satisfied result, the following protocol tips are strongly recommended. Scrap the cells from cell culture flasks with SDS loading buffer and boil them for 10 minutes immediately. Then prepare membranes with this freshly made lysate. Incubate the membrane with ab236626 at the dilution of 1/500 for 3 hours at room temperature and then secondary antibody ab97051 at the dilution of 1/20000 overnight. Acquire WB image with ECL substrate of high sensitivity.

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution

Lane 1:

Fresh 293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Fresh A549 (Human lung carcinoma epithelial cell) whole cell lysate at 40 µg

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20911-169

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB214815

Anti-TIFA (phospho T9) antibody [EPR19853]

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p>To get stronger band in western blot, we recommend using freshly made lysate to avoid degradation. Use SDS loading buffer to scrape cell from cell culture plate and boil cell lysate 10 minutes immediately. Incubate primary antibody for 3 hours at room temperature room and incubate secondary antibody overnight. Use higher sensitivity ECL substrate and exposure for longer time.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed : 28222186, PubMed : 28877472, PubMed : 30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed : 30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (PubMed : 30111836, PubMed : 35868845, PubMed : 38060563). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed : 27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed : 15883161). May play a role in ciliogenesis (PubMed : 30967659).
See full target information ALPK1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 642:201-211 PubMed40269158

2025

Microbial metabolite drives ageing-related clonal haematopoiesis via ALPK1.

Applications

Unspecified application

Species

Unspecified reactive species

Puneet Agarwal,Avery Sampson,Kathleen Hueneman,Kwangmin Choi,Niels Asger Jakobsen,Emma Uible,Chiharu Ishikawa,Jennifer Yeung,Lyndsey Bolanos,Xueheng Zhao,Kenneth D Setchell,David B Haslam,Jessica Galloway-Pena,John C Byrd,Paresh Vyas,Daniel T Starczynowski

iScience 28:111563 PubMed39868044

2025

IFN-γ licenses normal and pathogenic ALPK1/TIFA pathway in human monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Martin,Solène Caron,Mélissa Marcotte,Pauline Bronnec,Etienne Garneret,Nora Martel,Georgina Maalouf,Pascal Sève,David Saadoun,Yvan Jamilloux,Thomas Henry

Proceedings of the National Academy of Sciences of the United States of America 120:e2313148120 PubMed38060563

2023

ALPK1 mutants causing ROSAH syndrome or Spiradenoma are activated by human nucleotide sugars.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Snelling,Anton Saalfrank,Nicola T Wood,Philip Cohen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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