Rabbit Recombinant Monoclonal ALPK1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr20911-169-ab236626--western-blot-img74514.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)")
![Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr20911-169-ab236626--western-blot-img433205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)")
Publications
Filter by
- Proceedings of the National Academy of Sciences of the United States of America 120:e23131481202023ALPK1 mutants causing ROSAH syndrome or Spiradenoma are activated by human nucleotide sugars.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTom Snelling,Anton Saalfrank,Nicola T Wood,Philip CohenPubMed 38060563
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes To get stronger band in western blot, we recommend using freshly made lysate to avoid degradation. Use SDS loading buffer to scrape cell from cell culture plate and boil cell lysate 10 minutes immediately. Incubate primary antibody for 3 hours at room temperature room and incubate secondary antibody overnight. Use higher sensitivity ECL substrate and exposure for longer time. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed:28222186, PubMed:28877472, PubMed:30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed:30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (PubMed:30111836). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed:27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed:15883161). May play a role in ciliogenesis (PubMed:30967659).
Alternative names
KIAA1527, LAK, ALPK1, Alpha-protein kinase 1, Chromosome 4 kinase, Lymphocyte alpha-protein kinase
Recommended products
Rabbit Recombinant Monoclonal ALPK1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20911-169
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr20911-169-ab236626--western-blot-img74514.jpg "Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)")
Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.
Exposure time: 5 minutes
All lanes: Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution
Lane 1: ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg
Lane 2: Wild-type 293T whole cell lysate at 10 µg
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 139 kDa
Observed band size: 140 kDa
![Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr20911-169-ab236626--western-blot-img433205.jpg "Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)")
Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)
Blocking and Diluting buffer: 5% NFDM /TBST.
To get satisfied result, the following protocol tips are strongly recommended. Scrap the cells from cell culture flasks with SDS loading buffer and boil them for 10 minutes immediately. Then prepare membranes with this freshly made lysate. Incubate the membrane with ab236626 at the dilution of 1/500 for 3 hours at room temperature and then secondary antibody Goat Anti-Rabbit IgG H&L (HRP) ab97051 at the dilution of 1/20000 overnight. Acquire WB image with ECL substrate of high sensitivity.
All lanes: Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution
Lane 1: Fresh 293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg
Lane 2: Fresh A549 (Human lung carcinoma epithelial cell) whole cell lysate at 40 µg
Predicted band size: 139 kDa
Observed band size: 140 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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