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AB280366

Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free

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Rabbit Recombinant Monoclonal ALPK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA1527, LAK, ALPK1, Alpha-protein kinase 1, Chromosome 4 kinase, Lymphocyte alpha-protein kinase

2 Images
Western blot - Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (AB280366)
  • WB

Collaborator

Western blot - Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (AB280366)

This data was developed using ab236626, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.

Exposure time : 5 minutes

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (<a href='/en-us/products/primary-antibodies/alpk1-antibody-epr20911-169-ab236626'>ab236626</a>) at 1/500 dilution

Lane 1:

ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

Wild-type 293T whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Western blot - Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (AB280366)
  • WB

Lab

Western blot - Anti-ALPK1 antibody [EPR20911-169] - BSA and Azide free (AB280366)

Blocking and Diluting buffer : 5% NFDM /TBST.

To get satisfied result, the following protocol tips are strongly recommended. Scrap the cells from cell culture flasks with SDS loading buffer and boil them for 10 minutes immediately. Then prepare membranes with this freshly made lysate. Incubate the membrane with ab236626 at the dilution of 1/500 for 3 hours at room temperature and then secondary antibody ab97051 at the dilution of 1/20000 overnight. Acquire WB image with ECL substrate of high sensitivity.

This data was developed using ab236626, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (<a href='/en-us/products/primary-antibodies/alpk1-antibody-epr20911-169-ab236626'>ab236626</a>) at 1/500 dilution

Lane 1:

Fresh 293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Fresh A549 (Human lung carcinoma epithelial cell) whole cell lysate at 40 µg

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20911-169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab280366 is the carrier-free version of ab236626.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed : 28222186, PubMed : 28877472, PubMed : 30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed : 30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (PubMed : 30111836, PubMed : 35868845, PubMed : 38060563). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed : 27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed : 15883161). May play a role in ciliogenesis (PubMed : 30967659).
See full target information ALPK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com