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AB317808

Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free

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Knockout Tested Rabbit Recombinant Monoclonal ALPK1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Kiaa1527, Alpha-protein kinase 1

1 Images
Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (AB317808)
  • WB

Supplier Data

Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (AB317808)

This data was developed using ab317807, the same antibody clone in a different buffer formulation.

In western blot, ab317807 was shown to bind specifically to ALPK1. Target of interest was observed at 140 kDa in wild-type iBMM cell lysates (lane 1) with no signal observed at this size in ALPK1 knockout cell line (lane 2).

The identity of the bands below the target band are unknown.

The wild-type iBMM and ALPK1 knockout iBMM lysates were kindly provided by Dr Feng Shao's lab, NIBS.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ALPK1 antibody [EPR25006-61] (<a href='/en-us/products/primary-antibodies/alpk1-antibody-epr25006-61-ab317807'>ab317807</a>) at 1/1000 dilution

Lane 1:

Wild-type iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate at 50 µg

Lane 2:

ALPK1 knockout iBMM whole cell lysate at 50 µg

Lane 3:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg

Lane 4:

Mouse spleen tissue lysate at 50 µg

Lane 5:

Mouse thymus tissue lysate at 50 µg

Lane 6:

Mouse testis tissue lysate at 50 µg

Lane 7:

P388D1 (mouse lymphoma monocyte; macrophage) whole cell lysate at 50 µg

Lane 8:

LADMAC (mouse bone marrow monocyte macrophage) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,124 kDa

false

Exposure time: 136s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25006-61

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects clear signal in iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate.

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Reactivity data

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Product details

ab317808 is the carrier-free version of ab317807.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (By similarity). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed : 30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (By similarity). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (By similarity). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (By similarity). May play a role in ciliogenesis (By similarity).
See full target information Alpk1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com