Knockout Tested Rabbit Recombinant Monoclonal ALPK1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (AB317808), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr25006-61-bsa-and-azide-free-ab317808--western-blot-img432563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (AB317808)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | ICC/IF | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (By similarity). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed:30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (By similarity). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (By similarity). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (By similarity). May play a role in ciliogenesis (By similarity).
Alternative names
Kiaa1527, Alpha-protein kinase 1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal ALPK1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25006-61
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody only detects clear signal in iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317808 is the carrier-free version of Anti-ALPK1 antibody [EPR25006-61] ab317807.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (ab317808), expandable thumbnail](https://content.abcam.com/products/images/alpk1-antibody-epr25006-61-bsa-and-azide-free-ab317808--western-blot-img432563.jpg "Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (ab317808)")
Western blot - Anti-ALPK1 antibody [EPR25006-61] - BSA and Azide free (ab317808)
ALPK1 Western blot staining using rabbit Anti-ALPK1 antibody
This data was developed using Anti-ALPK1 antibody [EPR25006-61] ab317807, the same antibody clone in a different buffer formulation.
In western blot, Anti-ALPK1 antibody [EPR25006-61] ab317807 was shown to bind specifically to ALPK1. Target of interest was observed at 140 kDa in wild-type iBMM cell lysates (lane 1) with no signal observed at this size in ALPK1 knockout cell line (lane 2).
The identity of the bands below the target band are unknown.
The wild-type iBMM and ALPK1 knockout iBMM lysates were kindly provided by Dr Feng Shao's lab, NIBS.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-ALPK1 antibody [EPR25006-61] (Anti-ALPK1 antibody [EPR25006-61] ab317807) at 1/1000 dilution
Lane 1: Wild-type iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate at 50 µg with NFDM/TBST
Lane 2: ALPK1 knockout iBMM whole cell lysate at 50 µg with NFDM/TBST
Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg with NFDM/TBST
Lane 4: Mouse spleen tissue lysate at 50 µg with NFDM/TBST
Lane 5: Mouse thymus tissue lysate at 50 µg with NFDM/TBST
Lane 6: Mouse testis tissue lysate at 50 µg with NFDM/TBST
Lane 7: P388D1 (mouse lymphoma monocyte; macrophage) whole cell lysate at 50 µg with NFDM/TBST
Lane 8: LADMAC (mouse bone marrow monocyte macrophage) whole cell lysate at 50 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 140 kDa, 124 kDa
Exposure time: 136s
Downloads
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