Rabbit Polyclonal ALPPL2 antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Transfected cell line samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALPG aa 1-400.
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Publications
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- Cell reports 30:3917-3931.e52020Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Bi,Zhifen Tu,Yanping Zhang,Peng Yang,Mingyue Guo,Xuehao Zhu,Chengchen Zhao,Jianfeng Zhou,Hong Wang,Yixuan Wang,Shaorong GaoPubMed 32187559
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALPG aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P10696
Consider this alternative
Reactivity data
Select an application| ICC/IF | IHC-P | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Transfected cell line | Not recommended | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Transfected cell line | Dilution info 1/500 - 1/5000 | Notes - |
Target data
Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Alternative names
ALPPL, ALPPL2, ALPG, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like, GCAP, PLAP-like
Recommended products
Rabbit Polyclonal ALPPL2 antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Transfected cell line samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALPG aa 1-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALPG aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P10696
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-ALPPL2 antibody (ab96497)
Samples were separated by 10% SDS-PAGE.
All lanes: Western blot - Anti-ALPPL2 antibody (ab96497) at 1/5000 dilution
Lane 1: Non-transfected (–) 293 whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPPL2 antibody (ab96497)
Paraffin-embedded human Breast tissue stained for ALPPL2 protein with ab96497 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence - Anti-ALPPL2 antibody (ab96497)
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling ALPPL2 using ab96497 at a 1/1000 dilution at RT for 15 min (Green). Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000.The nuclear counter stain is Hoechst 33342 (Blue).
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