Anti-ALPPL2 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPPL2 antibody (AB96497)

Paraffin-embedded human Breast tissue stained for ALPPL2 protein with ab96497 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ALPPL2 antibody (AB96497)

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling ALPPL2 using ab96497 at a 1/1000 dilution at RT for 15 min (Green). Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000.The nuclear counter stain is Hoechst 33342 (Blue).

• WB

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Western blot - Anti-ALPPL2 antibody (AB96497)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALPPL2 antibody (ab96497) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293 whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

• WB

Supplier Data

Western blot - Anti-ALPPL2 antibody (AB96497)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ALPPL2 antibody (ab96497) at 1/5000 dilution

Lane 1:

AsPC-1 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false