Anti-Als2 antibody [EPR11185] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] - BSA and Azide free (AB249574)

This data was developed using ab170896, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Als2 with ab170896 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-Als2 antibody [EPR11185] - BSA and Azide free (AB249574)

This data was developed using the same antibody clone in a different buffer formulation (ab170896)

Immunoprecipitation of ALS2 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of ab170896 pre-coupled to Protein A beads. Samples were washed and processed for western blot with ab170896 at 1/1000

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

Lane 1:

Immunoprecipitation - Anti-Als2 antibody [EPR11185] (<a href='/en-us/products/primary-antibodies/als2-antibody-epr11185-ab170896'>ab170896</a>) at 1/1000 dilution

Lane 3:

Immunoprecipitation - Anti-Als2 antibody [EPR11185] (<a href='/en-us/products/primary-antibodies/als2-antibody-epr11185-ab170896'>ab170896</a>) at 2 µg

All lanes:

HAP1 cells

Observed band size: 184 kDa

false

• WB

Supplier Data

Western blot - Anti-Als2 antibody [EPR11185] - BSA and Azide free (AB249574)

This data was developed using ab170896, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Als2 antibody [EPR11185] (<a href='/en-us/products/primary-antibodies/als2-antibody-epr11185-ab170896'>ab170896</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 184 kDa

false

• WB

Lab

Western blot - Anti-Als2 antibody [EPR11185] - BSA and Azide free (AB249574)

This data was developed using the same antibody clone in a different buffer formulation (ab170896)

ab170896 was shown to react with ALS2 in wild-type HAP1 cells in Western blot with loss of signal observed in a ALS2 knockout cell line. Wild-type HAP1 and ALS2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab170896 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Als2 antibody [EPR11185] (<a href='/en-us/products/primary-antibodies/als2-antibody-epr11185-ab170896'>ab170896</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

ALS2 knock-out HAP1 lysate at 20 µg

Observed band size: 184 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] - BSA and Azide free (AB249574)

This data was developed using ab170896, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Als2 with ab170896 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.