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Rabbit Recombinant Monoclonal ALS2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (AB316196), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (AB316196), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (AB316196), expandable thumbnail
  • Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (AB316196), expandable thumbnail
  • Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (AB316196), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.

Alternative names

Alsin, Amyotrophic lateral sclerosis 2 protein homolog, b1309, JW1302, ggaP, ycjM

Recommended products

Rabbit Recombinant Monoclonal ALS2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27469-29
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316196 is the carrirer-free version of Anti-Als2 antibody [EPR27469-29] ab316195.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Als2 also known as ALS2 or Alsin is a protein implicated in intracellular trafficking. It contains a massive molecular size of approximately 184 kDa. Als2 exhibits multiple domains such as the VPS9 Dbl/CDC24 and ALS2CR domain which facilitate its role in regulating endosome dynamics. This protein expresses strongly in the nervous system especially within motor neurons and is also found in other tissues like the liver. Though Als2 functions largely within cellular compartments its presence in the central nervous system suggests a significant neural relevance.

Biological function summary

Als2 plays an important role in neuronal survival and function. It operates as part of a molecular complex involved in endosomal trafficking and membrane exchange. Als2 interacts with Rab5 a small GTPase facilitating early endosome membrane fusion. By acting on cellular vesicle pathways Als2 contributes critically to maintaining cellular homeostasis and membrane turnover in neuronal cells thereby supporting nervous system integrity.

Pathways

Als2 is essential in endocytic pathways and the regulation of synaptic vesicle cycling. Within these pathways Als2 interacts closely with other proteins such as Rab5 and Rac1 influencing cellular processes like endosome fusion and actin cytoskeleton remodeling. This interaction highlights its participation in organizing intracellular vesicle transport and contributing to the maintenance of synaptic function.

Associated diseases and disorders

Als2 has a direct association with neurodegenerative conditions particularly amyotrophic lateral sclerosis (ALS) and infantile-onset ascending hereditary spastic paralysis (IAHSP). Mutations in the Als2 gene disrupt normal protein function contributing to motor neuron degeneration and neuronal dysfunction. This relationship with neurodegenerative diseases highlights Als2's importance in motor neuron vitality. Additionally Als2 connects to other proteins such as SOD1 and TDP-43 in these conditions linking it to broader pathways implicated in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196)

    This data was developed using Anti-Als2 antibody [EPR27469-29] ab316195, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Als2 with Anti-Als2 antibody [EPR27469-29] ab316195 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: weak staining on mouse lung.

    The section was incubated with Anti-Als2 antibody [EPR27469-29] ab316195 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196)

    This data was developed using Anti-Als2 antibody [EPR27469-29] ab316195, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Als2 with Anti-Als2 antibody [EPR27469-29] ab316195 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with Anti-Als2 antibody [EPR27469-29] ab316195 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196)

    This data was developed using Anti-Als2 antibody [EPR27469-29] ab316195, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Als2 with Anti-Als2 antibody [EPR27469-29] ab316195 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebellum.

    The section was incubated with Anti-Als2 antibody [EPR27469-29] ab316195 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196), expandable thumbnail

    Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196)

    This data was developed using Anti-Als2 antibody [EPR27469-29] ab316195, the same antibody clone in a different buffer formulation.

    Low expression: mouse colon, mouse lung (PMID: 12837691; PMID: 11586298).

    The double bands observed are consistent with what has been described in the literature (PMID: 12837691).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Als2 antibody [EPR27469-29] (Anti-Als2 antibody [EPR27469-29] ab316195) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Mouse colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 160 kDa, 124 kDa

    Exposure time: 37s

  • Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196), expandable thumbnail

    Western blot - Anti-Als2 antibody [EPR27469-29] - BSA and Azide free (ab316196)

    This data was developed using Anti-Als2 antibody [EPR27469-29] ab316195, the same antibody clone in a different buffer formulation.

    The double bands observed are consistent with what has been described in the literature (PMID: 12837691).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Als2 antibody [EPR27469-29] (Anti-Als2 antibody [EPR27469-29] ab316195) at 1/1000 dilution

    Lane 1: HT-22 (mouse hippocampal neuronal cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 160 kDa, 124 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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