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AB180142

Anti-ALS2CR8 antibody [EPR13712]

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(1 Publication)

Rabbit Recombinant Monoclonal ALS2CR8 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ALS2CR8, CARF, Calcium-responsive transcription factor, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein, Calcium-response factor, Testis development protein NYD-SP24, CaRF

2 Images
Flow Cytometry (Intracellular) - Anti-ALS2CR8 antibody [EPR13712] (AB180142)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALS2CR8 antibody [EPR13712] (AB180142)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling ALS2CR8 with ab180142 at 1/90. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG

Western blot - Anti-ALS2CR8 antibody [EPR13712] (AB180142)
  • WB

Supplier Data

Western blot - Anti-ALS2CR8 antibody [EPR13712] (AB180142)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ALS2CR8 antibody [EPR13712] (ab180142) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13712

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence.
See full target information CARF

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 26:503 PubMed37822583

2023

Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Gu,Yongqing Ye,Rauniyar Sunil,Wenjian Zhan,Rutong Yu
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com