AB181101

Anti-Alx1 antibody [EPR11331]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal ALX1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

CART1, ALX1, ALX homeobox protein 1, Cartilage homeoprotein 1, CART-1

3 Images

Flow Cytometry (Intracellular) - Anti-Alx1 antibody [EPR11331] (AB181101)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alx1 antibody [EPR11331] (AB181101)

Intracellular Flow Cytometry analysis of 2% paraformaldehydefixed MCF7 cells labeling Alx1 with ab181101 at 1/230 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/50 dilution.

Supplier Data

Western blot - Anti-Alx1 antibody [EPR11331] (AB181101)

All lanes:

Western blot - Anti-Alx1 antibody [EPR11331] (ab181101) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-Alx1 antibody [EPR11331] (AB181101)

All lanes:

Western blot - Anti-Alx1 antibody [EPR11331] (ab181101) at 1/2000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

Carrier free

Anti-Alx1 antibody [EPR11331] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11331

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/230", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alx1 also known as aristaless-related homeobox 1 is a protein functioning as a transcription factor with an approximate mass of 41 kDa. Alx1 plays a critical role in gene expression regulation during embryonic development. It is expressed in various tissues particularly in craniofacial regions and influences the patterning of facial structures. Researchers have identified its involvement in skeletal morphogenesis linking it to the development and differentiation of tissues involved in forming the skeletal framework.

Biological function summary

Alx1 regulates the expression of genes involved in osteogenesis and craniofacial development. It acts as part of a larger transcriptional complex that guides cellular differentiation. This protein demonstrates its function by activating various downstream targets essential for normal bone and cartilage formation. Additionally Alx1's activity in cellular signaling pathways ensures proper tissue patterning during embryogenesis.

Pathways

Alx1 plays a significant role in the Wnt signaling and BMP (Bone Morphogenetic Protein) pathways both paramount to developmental processes. These pathways influence cell growth differentiation and apoptosis. Alx1 interacts with proteins such as β-catenin in the Wnt pathway which are vital for craniofacial formation. In the BMP pathway it interfaces with SMAD proteins facilitating transcription of osteogenic genes.

Alx1 mutations have links to facial dysostosis and developmental delays. These conditions stem from misregulated osteogenic pathways where Alx1 functionality is compromised. Alx1 has connections to other proteins like PAX9 which also plays roles in craniofacial development sharing pathways that influence developmental accuracy. Understanding Alx1's function and its associated networks aids in diagnosing and potentially developing treatments for these developmental disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes (PubMed : 8756334, PubMed : 9753625). Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures. Early on in development, it plays a role in forebrain mesenchyme survival (PubMed : 20451171). May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1 (PubMed : 23288509).

See full target information ALX1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:235-249 PubMed38267537

2024

p300 nucleocytoplasmic shuttling underlies mTORC1 hyperactivation in Hutchinson-Gilford progeria syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Min Son,So Jung Park,Sophia Y Breusegem,Delphine Larrieu,David C Rubinsztein

Nature communications 14:378 PubMed36690674

2023

Structural mechanism of BRD4-NUT and p300 bipartite interaction in propagating aberrant gene transcription in chromatin in NUT carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Di Yu,Yingying Liang,Claudia Kim,Anbalagan Jaganathan,Donglei Ji,Xinye Han,Xuelan Yang,Yanjie Jia,Ruirui Gu,Chunyu Wang,Qiang Zhang,Ka Lung Cheung,Ming-Ming Zhou,Lei Zeng

International journal of medical sciences 18:2510-2520 PubMed34104082

2021

Promoter Methylation-mediated Silencing of the MiR-192-5p Promotes Endometrial Cancer Progression by Targeting ALX1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjiao Ni,Wenjuan Tian,Shanhui Liang,Huaying Wang,Yulan Ren

Science signaling 13: PubMed33293463

2020

A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses.

Applications

Unspecified application

Species

Unspecified reactive species

Leah M Rommereim,Ajay Suresh Akhade,Bhaskar Dutta,Carolyn Hutcheon,Nicolas W Lounsbury,Clifford C Rostomily,Ram Savan,Iain D C Fraser,Ronald N Germain,Naeha Subramanian

Reproductive toxicology (Elmsford, N.Y.) 91:59-73 PubMed31705956

2019

Abnormal retinal pigment epithelium melanogenesis as a major determinant for radiation-induced congenital eye defects.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Craenen,Mieke Verslegers,Livine Craeghs,Roel Quintens,Ann Janssen,Amelie Coolkens,Sarah Baatout,Lieve Moons,Mohammed Abderrafi Benotmane

Diabetes 64:2664-75 PubMed25720387

2015

Epigenetic Regulation of Placenta-Specific 8 Contributes to Altered Function of Endothelial Colony-Forming Cells Exposed to Intrauterine Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Emily K Blue,BreAnn M Sheehan,Zia V Nuss,Frances A Boyle,Caleb M Hocutt,Cassandra R Gohn,Kaela M Varberg,Jeanette N McClintick,Laura S Haneline

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alx1 antibody [EPR11331]

Flow Cytometry (Intracellular) - Anti-Alx1 antibody [EPR11331] (AB181101)

Western blot - Anti-Alx1 antibody [EPR11331] (AB181101)

Western blot - Anti-Alx1 antibody [EPR11331] (AB181101)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise