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AB250348

Anti-Alx1 antibody [EPR11331] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ALX1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CART1, ALX1, ALX homeobox protein 1, Cartilage homeoprotein 1, CART-1

3 Images
Flow Cytometry (Intracellular) - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)

This data was developed using ab181101, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed MCF7 cells labeling Alx1 with ab181101 at 1/230 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/50 dilution.

Western blot - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)
  • WB

Supplier Data

Western blot - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)

This data was developed using ab181101, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Alx1 antibody [EPR11331] (<a href='/en-us/products/primary-antibodies/alx1-antibody-epr11331-ab181101'>ab181101</a>) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

Western blot - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)
  • WB

Supplier Data

Western blot - Anti-Alx1 antibody [EPR11331] - BSA and Azide free (AB250348)

This data was developed using ab181101, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Alx1 antibody [EPR11331] (<a href='/en-us/products/primary-antibodies/alx1-antibody-epr11331-ab181101'>ab181101</a>) at 1/2000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11331

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250348 is the carrier-free version of ab181101.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alx1 also known as aristaless-related homeobox 1 is a protein functioning as a transcription factor with an approximate mass of 41 kDa. Alx1 plays a critical role in gene expression regulation during embryonic development. It is expressed in various tissues particularly in craniofacial regions and influences the patterning of facial structures. Researchers have identified its involvement in skeletal morphogenesis linking it to the development and differentiation of tissues involved in forming the skeletal framework.
Biological function summary

Alx1 regulates the expression of genes involved in osteogenesis and craniofacial development. It acts as part of a larger transcriptional complex that guides cellular differentiation. This protein demonstrates its function by activating various downstream targets essential for normal bone and cartilage formation. Additionally Alx1's activity in cellular signaling pathways ensures proper tissue patterning during embryogenesis.

Pathways

Alx1 plays a significant role in the Wnt signaling and BMP (Bone Morphogenetic Protein) pathways both paramount to developmental processes. These pathways influence cell growth differentiation and apoptosis. Alx1 interacts with proteins such as β-catenin in the Wnt pathway which are vital for craniofacial formation. In the BMP pathway it interfaces with SMAD proteins facilitating transcription of osteogenic genes.

Alx1 mutations have links to facial dysostosis and developmental delays. These conditions stem from misregulated osteogenic pathways where Alx1 functionality is compromised. Alx1 has connections to other proteins like PAX9 which also plays roles in craniofacial development sharing pathways that influence developmental accuracy. Understanding Alx1's function and its associated networks aids in diagnosing and potentially developing treatments for these developmental disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes (PubMed : 8756334, PubMed : 9753625). Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures. Early on in development, it plays a role in forebrain mesenchyme survival (PubMed : 20451171). May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1 (PubMed : 23288509).
See full target information ALX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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