AB246927

Anti-AMACR antibody

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(3 Publications)

Rabbit Polyclonal AMACR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMACR aa 200-300.

View Alternative Names

Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Paraffin-embedded human kidney tissue stained for AMACR using ab246927 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Paraffin-embedded human lymph node tissue stained for AMACR using ab246927 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-AMACR antibody (AB246927)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AMACR antibody (AB246927)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling AMACR using ab246927 at 4 μg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Paraffin-embedded human pancreas tissue stained for AMACR using ab246927 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Paraffin-embedded human liver tissue stained for AMACR using ab246927 at 1/200 dilution in immunohistochemical analysis.

Lab

Western blot - Anti-AMACR antibody (AB246927)

Western blot : Rabbit Polyclonal to AMACR ab246927 staining at 0.4 µg/mL, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in AMACR knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AMACR antibody (ab246927) at 0.4 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

AMACR knockout U-87 MG at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 40 kDa,77 kDa

false

Unknown

Western blot - Anti-AMACR antibody (AB246927)

All lanes:

Western blot - Anti-AMACR antibody (ab246927) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 42 kDa

false

Unknown

Western blot - Anti-AMACR antibody (AB246927)

All lanes:

Western blot - Anti-AMACR antibody (ab246927) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human AMACR aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UHK6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed : 10655068, PubMed : 11060359, PubMed : 7649182). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed : 10655068, PubMed : 11060359, PubMed : 7649182).

See full target information AMACR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:279 PubMed40713574

2025

DNAJC24 suppresses breast cancer malignancy and serves as a prognostic biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Meng,Wei Liu,Yulong Yang,Xiaorui Wang,Linlin Zhan,Yi Luo,Liwei Chen,Yu Wang,Guangtao Li,Yehui Shi,Yuchao He,Zhongsheng Tong,Hua Guo

Signal transduction and targeted therapy 8:175 PubMed37121942

2023

Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ziruoyu Wang,Yanan Li,Wensi Zhao,Shuai Jiang,Yuqi Huang,Jun Hou,Xuelu Zhang,Zhaoyu Zhai,Chen Yang,Jiaqi Wang,Jiying Zhu,Jianbo Pan,Wei Jiang,Zengxia Li,Mingliang Ye,Minjia Tan,Haowen Jiang,Yongjun Dang

Proteomics 22:e2200081 PubMed36059095

2022

Proteomics reveals MRPL4 as a high-risk factor and a potential diagnostic biomarker for prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qihuan Fu,Ruixia Hong,Hang Zhou,Ying Li,Xiu Liu,Jiaqi Gong,Xiaoyang Wang,Jiajia Chen,Haiying Ran,Liting Wang,Fang Li,Jiangbei Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMACR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Immunocytochemistry/ Immunofluorescence - Anti-AMACR antibody (AB246927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody (AB246927)

Western blot - Anti-AMACR antibody (AB246927)

Western blot - Anti-AMACR antibody (AB246927)

Western blot - Anti-AMACR antibody (AB246927)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise