Anti-AMACR antibody [AMACR/1864]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL

Lane 1:

Recombinant AMACR protein

Lane 2:

Human kidney lysate

Predicted band size: 42 kDa

false

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL

All lanes:

Human kidney tissue lysate

Predicted band size: 42 kDa

false

• WB

Lab

Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)

Western blot : Mouse Monoclonal[AMACR/1864] to AMACR ab268062 staining at 2 µg/mL, shown in green; Rabbit anti-ACTN4 (ab108198) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in AMACR knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

AMACR knockout U-87 MG at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Mouse 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 40 kDa,100 kDa

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• Protein Array

Unknown

Protein Array - Anti-AMACR antibody [AMACR/1864] (AB268062)

Protein Array containing more than 19,000 full-length human proteins using ab268062. Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL

All lanes:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 42 kDa

false