Mouse Monoclonal AMACR antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AMACR aa 250 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27949.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062)")
![Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img8231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26371.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (AB268062)")
![Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img13884.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)")
![Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img90513.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMACR antibody [AMACR/1864] (AB268062)")
Publications
Filter by
- Signal transduction and targeted therapy 8:1752023Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiruoyu Wang,Yanan Li,Wensi Zhao,Shuai Jiang,Yuqi Huang,Jun Hou,Xuelu Zhang,Zhaoyu Zhai,Chen Yang,Jiaqi Wang,Jiying Zhu,Jianbo Pan,Wei Jiang,Zengxia Li,Mingliang Ye,Minjia Tan,Haowen Jiang,Yongjun DangPubMed 37121942
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human AMACR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHK6
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:10655068, PubMed:11060359, PubMed:7649182). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:10655068, PubMed:11060359, PubMed:7649182).
Alternative names
Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR
Recommended products
Mouse Monoclonal AMACR antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AMACR aa 250 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human AMACR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHK6
- Clone number
- AMACR/1864
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor concentrate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27949.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.
![Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img8231.jpg "Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
All lanes: Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL
All lanes: PC-3 (human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26371.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.
![Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img13884.jpg "Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
All lanes: Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL
All lanes: Human kidney tissue lysate
Predicted band size: 42 kDa
![Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--western-blot-img90513.jpg "Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
All lanes: Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/mL
Lane 1: Recombinant AMACR protein
Lane 2: Human kidney lysate
Predicted band size: 42 kDa
![Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--protein-array-img140509.jpg "Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)")
Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)
Protein Array containing more than 19,000 full-length human proteins using ab268062. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
![Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062), expandable thumbnail](https://content.abcam.com/products/images/amacr-antibody-amacr-1864-ab268062--protein-array-img140509.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)")
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