Anti-AMACR antibody [AMACR/1864] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (<a href='/en-us/products/primary-antibodies/amacr-antibody-amacr-1864-ab268062'>ab268062</a>) at 2 µg/mL

Lane 1:

Recombinant AMACR protein

Lane 2:

Human kidney lysate

Predicted band size: 42 kDa

false

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (<a href='/en-us/products/primary-antibodies/amacr-antibody-amacr-1864-ab268062'>ab268062</a>) at 2 µg/mL

All lanes:

Human kidney tissue lysate

Predicted band size: 42 kDa

false

• WB

Lab

Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

This data was developed using ab268062, the same antibody clone in a different buffer formulation.

Western blot : Mouse Monoclonal[AMACR/1864] to AMACR ab268062 staining at 2 µg/mL, shown in green; Rabbit anti-ACTN4 (ab108198) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in AMACR knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (<a href='/en-us/products/primary-antibodies/amacr-antibody-amacr-1864-ab268062'>ab268062</a>) at 2 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

AMACR knockout U-87 MG at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Mouse 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 40 kDa,100 kDa

false

• Protein Array

Unknown

Protein Array - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

Protein Array containing more than 19,000 full-length human proteins using ab268062.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

• WB

Unknown

Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (AB268183)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

All lanes:

Western blot - Anti-AMACR antibody [AMACR/1864] (<a href='/en-us/products/primary-antibodies/amacr-antibody-amacr-1864-ab268062'>ab268062</a>) at 2 µg/mL

All lanes:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 42 kDa

false