Anti-AMACR antibody - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody - BSA and Azide free (AB212378)

Immunohistochemical analysis of formalin fixed paraffin-embedded human prostate carcinoma tissue sections labeling AMACR with ab212378 at a 1/50 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody - BSA and Azide free (AB212378)

Immunohistochemical analysis of paraffin embedded, formalin fixed human lung carcinoma tissue labeling AMACR with ab212378 at 1/50 dilution.

• WB

Lab

Western blot - Anti-AMACR antibody - BSA and Azide free (AB212378)

This data was developed using ab246927, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Polyclonal to AMACR ab246927 staining at 0.4 µg/mL, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in AMACR knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AMACR antibody (<a href='/en-us/products/primary-antibodies/amacr-antibody-ab246927'>ab246927</a>) at 0.4 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

AMACR knockout U-87 MG at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 40 kDa,77 kDa

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