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AB155950

Anti-AMBN antibody [EPR8317]

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(1 Publication)

Rabbit Recombinant Monoclonal AMBN antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Ameloblastin, AMBN

1 Images
Western blot - Anti-AMBN antibody [EPR8317] (AB155950)
  • WB

Supplier Data

Western blot - Anti-AMBN antibody [EPR8317] (AB155950)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. Exposure time : Lane 1 : 1 second. Lane 2 : 20 seconds. AMBN will migrate to higher MW size (PMID : 23782691), the MW is consistent with literature (PMID : 26070558).

Lane 1:

Western blot - Anti-AMBN antibody [EPR8317] (ab155950) at 1/1000 dilution

Lane 2:

Western blot - Anti-GST antibody [EPR4236] (<a href='/en-us/products/primary-antibodies/gst-antibody-epr4236-ab111947'>ab111947</a>) at 1/1000 dilution

All lanes:

Recombinant AMBN protein

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 100 kDa

false

  • Carrier free

    Anti-AMBN antibody [EPR8317] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8317

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in the mineralization and structural organization of enamel.
See full target information AMBN

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 559:211-216 PubMed29973724

2018

Kinase-controlled phase transition of membraneless organelles in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Arpan Kumar Rai,Jia-Xuan Chen,Matthias Selbach,Lucas Pelkmans
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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