Rabbit Recombinant Monoclonal AMBN antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-AMBN antibody [EPR8317] - BSA and Azide free (AB249205), expandable thumbnail](https://content.abcam.com/products/images/ambn-antibody-epr8317-bsa-and-azide-free-ab249205--western-blot-img409943.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMBN antibody [EPR8317] - BSA and Azide free (AB249205)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Involved in the mineralization and structural organization of enamel.
Alternative names
Ameloblastin, AMBN
Recommended products
Rabbit Recombinant Monoclonal AMBN antibody. Carrier free. Suitable for WB and reacts with Human samples.
Alternative names
Ameloblastin, AMBN
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR8317
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249205 is the carrier-free version of Anti-AMBN antibody [EPR8317] ab155950.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-AMBN antibody [EPR8317] - BSA and Azide free (ab249205), expandable thumbnail](https://content.abcam.com/products/images/ambn-antibody-epr8317-bsa-and-azide-free-ab249205--western-blot-img409943.jpg "Western blot - Anti-AMBN antibody [EPR8317] - BSA and Azide free (ab249205)")
Western blot - Anti-AMBN antibody [EPR8317] - BSA and Azide free (ab249205)
This data was developed using Anti-AMBN antibody [EPR8317] ab155950, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: Lane 1: 1 second. Lane 2: 20 seconds.
AMBN will migrate to higher MW size (PMID: 23782691), the MW is consistent with literature (PMID: 26070558).Lane 1: Western blot - Anti-AMBN antibody [EPR8317] (Anti-AMBN antibody [EPR8317] ab155950) at 1/1000 dilution
Lane 2: Western blot - Anti-GST antibody [EPR4236] (Anti-GST antibody [EPR4236] ab111947) at 1/1000 dilution
All lanes: Recombinant AMBN protein
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 100 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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