Rabbit Recombinant Monoclonal AMCase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-Fr | IHC-P | |
---|---|---|---|
Mouse | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/8000 | Notes Use ab93684 as antigen retrieval buffer. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
Acidic mammalian chitinase, AMCase, Lung-specific protein TSA1902, CHIA
Rabbit Recombinant Monoclonal AMCase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR19984
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 30 seconds.
Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].
Negative control: Mouse liver
Lane 1: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/5000 dilution
Lane 2: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution
Lane 1: Mouse stomach lysate at 10 µg
Lane 2: Mouse liver lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
This data was developed using ab207169, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 5 seconds; Lane 2: 30 seconds.
Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID: 11085997; 12971947).
Negative control: Mouse liver.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Strong positive staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse frozen stomach tissue labeling AMCase with ab207169 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Strong cytoplasm staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].
Nuclear counterstain: DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].
All lanes: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution
All lanes: Mouse salivary gland tissue lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 30s
This data was developed using ab207169, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID: 11085997 and 12971947).
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on alveolar epithelium cells of mouse lung is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: no staining on mouse liver. [PMID:11085997]
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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