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Rabbit Recombinant Monoclonal AMCase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-AMCase antibody [EPR19984] (AB207169), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (AB207169), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19984] (AB207169), expandable thumbnail
  • Western blot - Anti-AMCase antibody [EPR19984] (AB207169), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (AB207169), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

Tested
Tested

Species

Mouse

Dilution info

1/8000

Notes

Use ab93684 as antigen retrieval buffer.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal AMCase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19984

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Western blot - Anti-AMCase antibody [EPR19984] (ab207169)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 30 seconds.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].

    Negative control: Mouse liver

    Lane 1: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/5000 dilution

    Lane 2: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution

    Lane 1: Mouse stomach lysate at 10 µg

    Lane 2: Mouse liver lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 50 kDa

    This data was developed using ab207169, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds; Lane 2: 30 seconds.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID: 11085997; 12971947).

    Negative control: Mouse liver.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Strong positive staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19984] (ab207169)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse frozen stomach tissue labeling AMCase with ab207169 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Strong cytoplasm staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Western blot - Anti-AMCase antibody [EPR19984] (ab207169)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].

    All lanes: Western blot - Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution

    All lanes: Mouse salivary gland tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 50 kDa

    Exposure time: 30s

    This data was developed using ab207169, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID: 11085997 and 12971947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Cytoplasm staining on alveolar epithelium cells of mouse lung is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] (ab207169)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver. [PMID:11085997]

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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