Anti-AMCase antibody [EPR19984] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : no staining on mouse liver. [PMID : 11085997]

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on alveolar epithelium cells of mouse lung is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse frozen stomach tissue labeling AMCase with ab207169 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Strong cytoplasm staining on bottom gastric glands of mouse stomach is observed [PMID : 12971947]. Nuclear counterstain : DAPI (Blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong positive staining on bottom gastric glands of mouse stomach is observed [PMID : 12971947].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID : 11085997 and 12971947).

All lanes:

Western blot - Anti-AMCase antibody [EPR19984] (<a href='/en-us/products/primary-antibodies/amcase-antibody-epr19984-ab207169'>ab207169</a>) at 1/2000 dilution

All lanes:

Mouse salivary gland tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 50 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-AMCase antibody [EPR19984] - BSA and Azide free (AB251465)

This data was developed using ab207169, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 5 seconds; Lane 2 : 30 seconds.

Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon (PMID : 11085997; 12971947).

Negative control : Mouse liver.

Lane 1:

Western blot - Anti-AMCase antibody [EPR19984] (<a href='/en-us/products/primary-antibodies/amcase-antibody-epr19984-ab207169'>ab207169</a>) at 1/5000 dilution

Lane 2:

Western blot - Anti-AMCase antibody [EPR19984] (<a href='/en-us/products/primary-antibodies/amcase-antibody-epr19984-ab207169'>ab207169</a>) at 1/2000 dilution

Lane 1:

Mouse stomach lysate at 10 µg

Lane 2:

Mouse liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 50 kDa

false