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AB122312

Anti-Amelotin antibody

4

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal Amelotin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMTN aa 1-150.

View Alternative Names

UNQ689/PRO1329, AMTN, Amelotin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Amelotin antibody (AB122312)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Amelotin antibody (AB122312)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122312 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amelotin antibody (AB122312)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amelotin antibody (AB122312)

ab122312, at 1/150, staining Amelotin in paraffin embedded Human cerebellum tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AMTN aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q6UX39

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Amelotin also known as AMTN is a protein involved in the formation and mineralization of dental enamel. The protein has an estimated mass of approximately 24 kilodaltons. It is expressed in high amounts in ameloblasts which are cells involved in dental enamel production especially in the maturation stage of enamel development.
Biological function summary

The protein encoded by the Amelotin gene plays a significant role in dental epithelial cell differentiation and enamel biomineralization. Amelotin does not appear to form part of a larger protein complex but acts in coordination with other enamel matrix proteins. Its expression increases as ameloblasts transition into maturation stage suggesting it modulates enamel matrix mineralization.

Pathways

The protein influences tooth development processes particularly within the amelogenesis pathway. Amelotin interacts with and complements the roles of other enamel matrix proteins such as amelogenin and enamelin. Its activity contributes to the regulation of calcium and phosphate ion deposition required for enamel hardening.

Malfunction or altered expression of Amelotin associates with amelogenesis imperfecta a disorder affecting enamel development. The interaction between Amelotin and enamelin may be important in understanding the pathophysiology of this condition. It is also implicated in carious lesions due to its potential impact on enamel resilience. Understanding Amelotin's function can offer insights into these dental health issues and point to therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.
See full target information AMTN
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta biomaterialia 141:70-88 PubMed34971784

2022

Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas G Fischer,Alexandra C Kobe,Jinhong Dai,Jiahe He,Hongning Wang,John A Pizarek,David A De Jong,Zhou Ye,Shengbin Huang,Conrado Aparicio

Translational research : the journal of laboratory 219:45-62 PubMed32160961

2020

Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Dinusha Rajapakse,Katherine Peterson,Sanghamitra Mishra,Jianguo Fan,Joshua Lerner,Maria Campos,Graeme Wistow

Journal of oral science 60:388-398 PubMed30158339

2018

IL-1β and TNF-α regulate mouse amelotin gene transcription in gingival epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Noda,Mizuho Yamazaki,Yasunobu Iwai,Sari Matsui,Ayako Kato,Hideki Takai,Yohei Nakayama,Yorimasa Ogata

FEBS open bio 8:974-985 PubMed29928577

2018

Transcriptional regulation of human amelotin gene by interleukin-1β.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuho Yamazaki,Masaru Mezawa,Keisuke Noda,Yasunobu Iwai,Sari Matsui,Hideki Takai,Yohei Nakayama,Yorimasa Ogata

Journal of oral science 56:261-8 PubMed25500923

2014

Proinflammatory cytokines induce amelotin transcription in human gingival fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Nakayama,Hideki Takai,Sari Matsui,Hiroyoshi Matsumura,Liming Zhou,Ayako Kato,Bernhard Ganss,Yorimasa Ogata

PloS one 8:e84201 PubMed24376796

2013

Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans.

Applications

IHC-P

Species

Human

Hyo-Seol Lee,Jongeun Lee,Seong-Oh Kim,Je-Seon Song,Jae-Ho Lee,Syng-Ill Lee,Han-Sung Jung,Byung-Jai Choi
View all publications
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