Rabbit Polyclonal AMF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GPI.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GPI. The exact immunogen used to generate this antibody is proprietary information. Database link P06744
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Chimpanzee | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:11004567, PubMed:3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:11004567, PubMed:3352745).
Alternative names
Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36
Recommended products
Rabbit Polyclonal AMF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GPI.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GPI. The exact immunogen used to generate this antibody is proprietary information. Database link P06744
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-AMF antibody (ab227472)
7.5% SDS-PAGE
All lanes: Western blot - Anti-AMF antibody (ab227472) at 1/10000 dilution
Lane 1: NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody (ab227472)
Paraffin-embedded NCI-N87 xenograft tissue stained for AFM with ab227472 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody (ab227472)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AFM (green) using ab227472 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
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