Rabbit Polyclonal AMH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMH.
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Publications
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- EBioMedicine 90:1045352023Hypothalamic neuroglial plasticity is regulated by anti-Müllerian hormone and disrupted in polycystic ovary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne-Laure Barbotin,Nour El Houda Mimouni,Grégory Kuchcinski,Renaud Lopes,Romain Viard,Sowmyalakshmi Rasika,Daniele Mazur,Mauro S B Silva,Virginie Simon,Angèle Boursier,Jean-Pierre Pruvo,Qiang Yu,Michael Candlish,Ulrich Boehm,Federica Dal Bello,Claudio Medana,Pascal Pigny,Didier Dewailly,Vincent Prevot,Sophie Catteau-Jonard,Paolo GiacobiniPubMed 37001236
- Journal of clinical medicine 11:2022Protective Effect and Mechanism of Melatonin on Cisplatin-Induced Ovarian Damage in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFen Xing,Mengyao Wang,Zhiming Ding,Junhui Zhang,Simin Ding,Lingge Shi,Qinge Xie,Muhammad Jamil Ahmad,Zhaolian Wei,Liang Tang,Dan Liang,Yunxia Cao,Yajing LiuPubMed 36555999
- Reproductive biology and endocrinology : RB&E 19:1192021BPA and BPS affect the expression of anti-Mullerian hormone (AMH) and its receptor during bovine oocyte maturation and early embryo development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela Christina Saleh,Reem Sabry,Gabriela Fabiana Mastromonaco,Laura Alessandra FavettaPubMed 34344364
- Scientific reports 11:141392021Dysregulation of anti-Mullerian hormone expression levels in mural granulosa cells of FMR1 premutation carriers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoran Friedman-Gohas,Raoul Orvieto,Abigael Michaeli,Adva Aizer,Michal Kirshenbaum,Yoram CohenPubMed 34238973
- Journal of ovarian research 13:222020FMRpolyG accumulates in FMR1 premutation granulosa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Friedman-Gohas,S E Elizur,O Dratviman-Storobinsky,A Aizer,J Haas,H Raanani,R Orvieto,Y CohenPubMed 32101156
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AMH. The exact immunogen used to generate this antibody is proprietary information. Database link P03971
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays an important role in several reproductive functions. Induces Muellerian duct regression during male fetal sexual differentiation (PubMed:34155118, PubMed:3754790, PubMed:8469238). Also plays a role in Leydig cell differentiation and function (By similarity). In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed:14742691). AMH signals by binding to a specific type-II receptor, AMHR2, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A), and recruiting SMAD proteins that are translocated to the nucleus to regulate target gene expression (PubMed:20861221, PubMed:34155118).
Alternative names
MIF, AMH, Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance, MIS
Recommended products
Rabbit Polyclonal AMH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMH.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AMH. The exact immunogen used to generate this antibody is proprietary information. Database link P03971
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-AMH antibody - C-terminal (ab229212)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-AMH antibody - C-terminal (ab229212) at 1/2500 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (ab229212)
Paraffin-embedded mouse ovary tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (ab229212)
Paraffin-embedded mouse testis tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-AMH antibody - C-terminal (ab229212)
Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for AMH (green) using ab229212 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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