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AB229212

Anti-AMH antibody - C-terminal

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(10 Publications)

Rabbit Polyclonal AMH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMH.

View Alternative Names

MIF, AMH, Anti-Muellerian hormone, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-AMH antibody - C-terminal (AB229212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AMH antibody - C-terminal (AB229212)

Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for AMH (green) using ab229212 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (AB229212)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (AB229212)

Paraffin-embedded mouse testis tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (AB229212)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (AB229212)

Paraffin-embedded mouse ovary tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-AMH antibody - C-terminal (AB229212)
  • WB

Supplier Data

Western blot - Anti-AMH antibody - C-terminal (AB229212)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-AMH antibody - C-terminal (ab229212) at 1/2500 dilution

All lanes:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AMH. The exact immunogen used to generate this antibody is proprietary information.

P03971

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 14742691, PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (PubMed : 20861221, PubMed : 34155118). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). Also plays a role in Leydig cell differentiation and function (By similarity).
See full target information AMH
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:30681 PubMed39730417

2024

Chronic unpredictable stress induces anxiety-like behavior and oxidative stress, leading to diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Zhou,Yangshuo Li,Jie Ding,Shuai Sun,Wen Cheng,Jin Yu,Zailong Cai,Zhexin Ni,Chaoqin Yu

Nutrients 16: PubMed39339685

2024

Effects of Melatonin and 3,5,3'-Triiodothyronine on the Development of Rat Granulosa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqi Wu,Yilin Yao,Rui Chen,Baoqiang Fu,Ying Sun,Yakun Yu,Yan Liu,Haoyuan Feng,Shuaitian Guo,Yanzhou Yang,Cheng Zhang

Proceedings of the National Academy of Sciences of the United States of America 121:e2312724121 PubMed38315849

2024

A massive alteration of gene expression in undescended testicles of dogs and the association of variants with cryptorchidism.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Stachowiak,Joanna Nowacka-Woszuk,Alicja Szabelska-Beresewicz,Joanna Zyprych-Walczak,Paulina Krzeminska,Oskar Sosinski,Tomasz Nowak,Marek Switonski

EBioMedicine 90:104535 PubMed37001236

2023

Hypothalamic neuroglial plasticity is regulated by anti-Müllerian hormone and disrupted in polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Laure Barbotin,Nour El Houda Mimouni,Grégory Kuchcinski,Renaud Lopes,Romain Viard,Sowmyalakshmi Rasika,Daniele Mazur,Mauro S B Silva,Virginie Simon,Angèle Boursier,Jean-Pierre Pruvo,Qiang Yu,Michael Candlish,Ulrich Boehm,Federica Dal Bello,Claudio Medana,Pascal Pigny,Didier Dewailly,Vincent Prevot,Sophie Catteau-Jonard,Paolo Giacobini

Journal of clinical medicine 11: PubMed36555999

2022

Protective Effect and Mechanism of Melatonin on Cisplatin-Induced Ovarian Damage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Xing,Mengyao Wang,Zhiming Ding,Junhui Zhang,Simin Ding,Lingge Shi,Qinge Xie,Muhammad Jamil Ahmad,Zhaolian Wei,Liang Tang,Dan Liang,Yunxia Cao,Yajing Liu

International journal of biological sciences 18:5168-5184 PubMed35982894

2022

Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ni,Guo-Hua Wu,Juan-Juan Cai,Run Zhang,Yang Zheng,Jing-Quan Liu,Xiang-Hong Yang,Xue Yang,Ye Shen,Jun-Mei Lai,Xiang-Ming Ye,Shi-Jing Mo

Evidence-based complementary and alternative medicine : eCAM 2022:2112359 PubMed35432561

2022

Baicalin Attenuates Continuous Activation of -Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Fan,Feng Dai,Chunhu Zhang,Lu Zhou,Xinjian Qiu,Yunhui Li

Reproductive biology and endocrinology : RB&E 19:119 PubMed34344364

2021

BPA and BPS affect the expression of anti-Mullerian hormone (AMH) and its receptor during bovine oocyte maturation and early embryo development.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Christina Saleh,Reem Sabry,Gabriela Fabiana Mastromonaco,Laura Alessandra Favetta

Scientific reports 11:14139 PubMed34238973

2021

Dysregulation of anti-Mullerian hormone expression levels in mural granulosa cells of FMR1 premutation carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Moran Friedman-Gohas,Raoul Orvieto,Abigael Michaeli,Adva Aizer,Michal Kirshenbaum,Yoram Cohen

Journal of ovarian research 13:22 PubMed32101156

2020

FMRpolyG accumulates in FMR1 premutation granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

M Friedman-Gohas,S E Elizur,O Dratviman-Storobinsky,A Aizer,J Haas,H Raanani,R Orvieto,Y Cohen
View all publications
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