Anti-AMH antibody [EPR23380-171] - BSA and Azide free

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

4% PFA-fixed, 0.2% Triton X-100 permeabilised rat ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of rat ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

4% PFA-fixed, 0.2% Triton X-100 permeabilised mouse ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of mouse ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

This data was developed using ab272221, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse ovary tissue staining AMH with ab272221 at a 1 : 2000 (0.26 μg/ml) dilution, ab182744 Collagen VI used at 1 : 2000 (0.76 ug/ml) dilution and ab270534 DDX4 / MVH used at 1 : 5000 (0.1 μg/ml) dilution.

Panel A : merged staining of anti-AMH (green; Opal™570), anti-Collagen VI (magenta; Opal™690) and anti-DDX4 / MVH (gray; Opal™570) on mouse ovary.

Panel B : anti-AMH staining granulosa cells in mouse ovary.

Panel C : ant-Collagen VI staining stroma in mouse ovary.

Panel D : ant-DDX4 / MVH staining oocytes in mouse ovary.

Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab272221, ab182744 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (AB272227)

This data was developed using ab272221, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat ovary tissue staining AMH with ab272221 at a 1 : 2000 (0.26 μg/ml) dilution, ab182744 Collagen VI used at 1 : 2000 (0.76 ug/ml) dilution and ab270534 DDX4 / MVH used at 1 : 5000 (0.1 μg/ml) dilution.

Panel A : merged staining of anti-AMH (green; Opal™570), anti-Collagen VI (magenta; Opal™690) and anti-DDX4 / MVH (gray; Opal™570) on rat ovary.

Panel B : anti-AMH staining granulosa cells in rat ovary.

Panel C : ant-Collagen VI staining stroma in rat ovary.

Panel D : ant-DDX4 / MVH staining oocytes in rat ovary.

Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab272221, ab182744 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.