Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)

Rabbit Recombinant Monoclonal AMH antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	IP
Human	Not recommended	Tested	Expected
Mouse	Tested	Tested	Tested
Rat	Tested	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Amh

Function

Plays an important role in several reproductive functions, including Muellerian duct regression during male fetal sexua,l differentiation and in the adult plays a role in Leydig cell differentiation and function (PubMed:1782869, PubMed:9435237). In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed:11606457, PubMed:11861535). Binds to its sole type II receptor, AMHR2 that recruits type I receptors ACVR1 and BMPR1A which subsequently activates the Smad pathway (By similarity).

Alternative names

Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance, AMH, MIS, Amh

Recommended products

Rabbit Recombinant Monoclonal AMH antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25835-27
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for human IHC-P.
Unsuitable for rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab313768 is the carrier-free version of Anti-AMH antibody [EPR25835-27] ab313767.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Negative control: spleen (PMID:1782869).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-AMH antibody [EPR25835-27] (Anti-AMH antibody [EPR25835-27] ab313767) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human ovary tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 59 kDa
Exposure time: 136s
Western blot - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, skin (PMID:1782869).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 10 seconds
All lanes: Western blot - Anti-AMH antibody [EPR25835-27] (Anti-AMH antibody [EPR25835-27] ab313767) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse ovary tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse skin tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 59 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat skin. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat spleen. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat E14.5 testis tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat E14.5 testis. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse spleen. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 testis tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse E14.5 testis. (A) Low‑powered (magnification, x4) and (B) high‑powered (magnification, x20) microscopic images. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat ovary tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat ovarian granulosa cells. The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
AMH was immunoprecipitated from 0.35 mg mouse testis tissue lysate with Anti-AMH antibody [EPR25835-27] ab313767 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-AMH antibody [EPR25835-27] ab313767 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AMH antibody [EPR25835-27] ab313767 in mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
All lanes: Immunoprecipitation - Anti-AMH antibody [EPR25835-27] (Anti-AMH antibody [EPR25835-27] ab313767) at 1/30 dilution
All lanes: Mouse testis tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (ab313768)
This data was developed using Anti-AMH antibody [EPR25835-27] ab313767, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling AMH with Anti-AMH antibody [EPR25835-27] ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse ovarian granulosa cells (PMID: 16556768). The section was incubated with Anti-AMH antibody [EPR25835-27] ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.