Anti-AMH antibody [EPR25835-27] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling AMH with ab313767 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse spleen. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat spleen. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat skin. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat E14.5 testis tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat E14.5 testis. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse ovarian granulosa cells (PMID : 16556768). The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat ovary tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat ovarian granulosa cells. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse E14.5 testis tissue labeling AMH with ab313767 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse E14.5 testis. (A) Low‑powered (magnification, x4) and (B) high‑powered (magnification, x20) microscopic images. The section was incubated with ab313767 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. AMH was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab313767 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313767 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse testis tissue lysate Lane 2 : Mouse testis tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313767 in mouse testis tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-AMH antibody [EPR25835-27] (<a href='/en-us/products/primary-antibodies/amh-antibody-epr25835-27-ab313767'>ab313767</a>) at 1/30 dilution

All lanes:

Mouse testis tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Negative control : spleen (PMID : 1782869).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-AMH antibody [EPR25835-27] (<a href='/en-us/products/primary-antibodies/amh-antibody-epr25835-27-ab313767'>ab313767</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human ovary tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 59 kDa

true

Exposure time: 136s

• WB

Supplier Data

Western blot - Anti-AMH antibody [EPR25835-27] - BSA and Azide free (AB313768)

This data was developed using ab313767, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen, skin (PMID : 1782869). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-AMH antibody [EPR25835-27] (<a href='/en-us/products/primary-antibodies/amh-antibody-epr25835-27-ab313767'>ab313767</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 59 kDa

false

Exposure time: 10s