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AB302674

Anti-AMID antibody [6D8] - BSA and Azide free

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Rat Recombinant Monoclonal AMID antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

View Alternative Names

AMID, PRG3, AIFM2, Ferroptosis suppressor protein 1, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, p53-responsive gene 3 protein

1 Images
Western blot - Anti-AMID antibody [6D8] - BSA and Azide free (AB302674)
  • WB

Supplier Data

Western blot - Anti-AMID antibody [6D8] - BSA and Azide free (AB302674)

This data was developed using ab302673, the same antibody clone in a different buffer formulation. ,The expression profile weight observed is consistent with what has been described in the literature (PMID : 31634899).Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 seconds

All lanes:

Western blot - Anti-AMID antibody [6D8] (<a href='/en-us/products/primary-antibodies/amid-antibody-6d8-ab302673'>ab302673</a>) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell), whole cell lysate 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 µg

Lane 3:

A549 (human lu carcinoma epithelial cell), whole cell lysate 20 µg

Lane 4:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Observed band size: 40 kDa

false

Exposure time: 3s

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

6D8

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography DEAE-C
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis (PubMed : 31634899, PubMed : 31634900, PubMed : 35922516). At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis (PubMed : 31634899, PubMed : 31634900). Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis (PubMed : 31634899, PubMed : 31634900). This anti-ferroptotic function is independent of cellular glutathione levels (PubMed : 31634899, PubMed : 31634900). Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle : catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms (PubMed : 35922516). Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis (PubMed : 35922516). May play a role in mitochondrial stress signaling (PubMed : 26689472). Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed : 26689472). Capable of DNA binding in a non-sequence specific way (PubMed : 15958387).
See full target information AIFM2
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Product promise

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