JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB76124

Anti-Amphiphysin antibody [EP2061Y]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Amphiphysin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

AMPH1, AMPH, Amphiphysin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2061Y] (AB76124)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue with ab76124 at 1/5000 (0.23 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab76124 for 30 mins at room temperature.

Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-Amphiphysin antibody [EP2061Y] (AB76124)
  • WB

Lab

Western blot - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

Exposure time : Lane 1, 20 seconds; Lane 2, 10 seconds; Lane 3, 5 seconds.

All lanes:

Western blot - Anti-Amphiphysin antibody [EP2061Y] (ab76124) at 1/100000 dilution

Lane 1:

Human brain lysate at 15 µg with 5% NFDM/TBST

Lane 2:

Mouse brain lysate at 15 µg with 5% NFDM/TBST

Lane 3:

Rat brain lysate at 15 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 128 kDa

false

  • Carrier free

    Anti-Amphiphysin antibody [EP2061Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2061Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

The production method for this product has been changed from hybridoma to recombniant on 16th April 2024

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Amphiphysin also known as BIN1 or PHIPBP1 functions mechanically as a protein implicated in endocytosis. It has a molecular mass around 128 kDa. You find it expressed in various tissues but most notably in the brain and muscle where it plays essential roles in synaptic vesicle recycling. This protein has a BAR domain that allows it to bind to lipid membranes facilitating membrane curvature necessary for vesicle budding in neurons.
Biological function summary

In many cellular contexts Amphiphysin acts in forming a heterodimer complex with Dynamin which is necessary for synaptic vesicle endocytosis. The protein serves a role in membrane dynamics and is involved in the scission of newly formed vesicles from the plasma membrane. Using Amphiphysin antibodies can help detect or isolate this protein aiding in the study of synaptic transmission and neuromuscular functions.

Pathways

The protein plays a role in clathrin-mediated endocytosis and interacts closely with the protein Clathrin. Amphiphysin antibodies help in studying these relationships as Amphiphysin modulates actin cytoskeleton organization. It also relates to the Dynamin family through its function in synaptic vesicle retrieval highlighting its central role within the synaptic function pathway.

Mutations or dysregulation of Amphiphysin relate to conditions like Stiff Person Syndrome and Barre-Liéou syndrome. These are neurological disorders where the malfunction of Amphiphysin adversely affects synaptic function and it may interact with GABAergic pathways. Anti-amphiphysin antibodies can be used in diagnostics to identify autoimmune response in these disorders showing the protein's significance in maintaining proper neurological health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
See full target information AMPH
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB244375

Anti-Amphiphysin antibody

primary-antibodies

amphiphysin-antibody-ab244375

0

(0 reviews)

Alternative Product
Primary Antibodies

AB52646

Anti-Amphiphysin antibody [EP2060Y]

primary-antibodies

amphiphysin-antibody-ep2060y-ab52646

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com