AB180722

Anti-Amphiregulin antibody

Name: Anti-Amphiregulin antibody (ab180722) | Abcam
Brand: Abcam Limited
SKU: AB180722
Rating: 4 (3 reviews)

(3 Reviews)

(4 Publications)

Rabbit Polyclonal Amphiregulin antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AREG.

View Alternative Names

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

3 Images

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

Blocking buffer : 3% nonfat dry milk in TBST. PMID : 9642297.

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution

All lanes:

A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

Blocking buffer : 3% nonfat dry milk in TBST. PMID : 9642297.

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution

All lanes:

Mouse brain lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Exposure time: 300s

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/500 dilution

Lane 1:

MCF7 cell extracts

Lane 2:

A549 cell extracts

Lane 3:

HT29 cell extracts

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AREG.

P15514

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.

Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

See full target information AREG

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 19:370 PubMed34789290

2021

Nickel nanoparticle-induced cell transformation: involvement of DNA damage and DNA repair defect through HIF-1α/miR-210/Rad52 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Mo,Yue Zhang,Yuanbao Zhang,Jiali Yuan,Luke Mo,Qunwei Zhang

iScience 24:103243 PubMed34746706

2021

Renal subcapsular delivery of PGE promotes kidney repair by activating endogenous Sox9 stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Chen,Haoyan Huang,Yue Liu,Chen Wang,Xiaoniao Chen,Yuqiao Chang,Yuhao Li,Zhikun Guo,Zhibo Han,Zhong-Chao Han,Qiang Zhao,Xiang-Mei Chen,Zongjin Li

Molecular medicine reports 24: PubMed34396425

2021

HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Geng,Guangli Liu,Jiandong Hu,Jianchao Li,Dong Wang,Shenshan Zou,Xintao Xu

Frontiers in oncology 10:160 PubMed32154166

2020

Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhua Dong,Zhen Yang,Hongwei Yang,Dongyan Li,Xinguang Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Amphiregulin antibody

Western blot - Anti-Amphiregulin antibody (AB180722)

Western blot - Anti-Amphiregulin antibody (AB180722)

Western blot - Anti-Amphiregulin antibody (AB180722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise