Rabbit Polyclonal Amphiregulin antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AREG.
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- iScience 24:1032432021Renal subcapsular delivery of PGE promotes kidney repair by activating endogenous Sox9 stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShang Chen,Haoyan Huang,Yue Liu,Chen Wang,Xiaoniao Chen,Yuqiao Chang,Yuhao Li,Zhikun Guo,Zhibo Han,Zhong-Chao Han,Qiang Zhao,Xiang-Mei Chen,Zongjin LiPubMed 34746706
- Molecular medicine reports 24:2021HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Geng,Guangli Liu,Jiandong Hu,Jianchao Li,Dong Wang,Shenshan Zou,Xintao XuPubMed 34396425
- Frontiers in oncology 10:1602020Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinhua Dong,Zhen Yang,Hongwei Yang,Dongyan Li,Xinguang QiuPubMed 32154166
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human AREG. Database link P15514
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
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Target data
Function
Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Alternative names
AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF
Recommended products
Rabbit Polyclonal Amphiregulin antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AREG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human AREG. Database link P15514
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
- Biological function summary
'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.
- Pathways
'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.
- Associated diseases and disorders
'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Amphiregulin antibody (ab180722)
All lanes: Western blot - Anti-Amphiregulin antibody (ab180722) at 1/500 dilution
Lane 1: MCF7 cell extracts
Lane 2: A549 cell extracts
Lane 3: HT29 cell extracts
Predicted band size: 28 kDa
Western blot - Anti-Amphiregulin antibody (ab180722)
Blocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.All lanes: Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution
All lanes: Mouse brain lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa
Exposure time: 300s
Western blot - Anti-Amphiregulin antibody (ab180722)
Blocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.All lanes: Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution
All lanes: A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa
Exposure time: 3s
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