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AB224350

Anti-Amphiregulin antibody

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(2 Publications)

Rabbit Polyclonal Amphiregulin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AREG aa 1-200.

View Alternative Names

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)

Paraffin-embedded human testis stained for Amphiregulin using ab224350 at 1/500 dilution in immunohistochemical analysis. Shows weak cytoplasmic and membranous positivity in cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)

Paraffin-embedded human Fallopian tube stained for Amphiregulin using ab224350 at 1/500 dilution in immunohistochemical analysis. Shows moderate cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)

Paraffin-embedded human liver stained for Amphiregulin using ab224350 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB224350)

Paraffin-embedded human placenta stained for Amphiregulin using ab224350 at 1/500 dilution in immunohistochemical analysis. Shows moderate cytoplasmic and membranous positivity in trophoblastic cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AREG aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P15514

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
See full target information AREG
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology :18676 PubMed37997936

2023

WITHDRAWN: Ethyl acetate extracted from the chinese medicinal plant Caesalpinia sappan L alleviates gustatory response in diabetes mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Cao,Li Cui,Wen Yang

Journal of virology 93: PubMed31434729

2019

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Eleonora Karagianni,Deepali Vasoya,Jeanie Finlayson,Henny M Martineau,Ann R Wood,Chris Cousens,Mark P Dagleish,Mick Watson,David J Griffiths
View all publications
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Product promise

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