Rabbit Recombinant Monoclonal Amphiregulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (AB244915), expandable thumbnail](https://content.abcam.com/products/images/amphiregulin-antibody-epr19233-156-bsa-and-azide-free-detector-ab244915--sandwich-elisa-img422710.png/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (AB244915)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Alternative names
AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF
Recommended products
Rabbit Recombinant Monoclonal Amphiregulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19233-156
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab244915 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Amphiregulin. The recommended pair for sandwich ELISA is:
Capture: Anti-Amphiregulin antibody [EPR18223-155] - BSA and Azide free (Capture) ab244653, Human Amphiregulin Capture Antibody (unconjugated)
Detector: ab244915, Human Amphiregulin Detector Antibody (unconjugated)
The reference range value is 18 - 600 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
- Biological function summary
'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.
- Pathways
'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.
- Associated diseases and disorders
'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (ab244915), expandable thumbnail](https://content.abcam.com/products/images/amphiregulin-antibody-epr19233-156-bsa-and-azide-free-detector-ab244915--sandwich-elisa-img422710.png "Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (ab244915)")
Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (ab244915)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Amphiregulin ELISA Kit ab222504).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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