AB207442

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]

RabMAb
Recombinant
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(50 Publications)

Rabbit Recombinant Monoclonal AMPK alpha 1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 50 publications.

View Alternative Names

AMPK1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase

5 Images

Supplier Data

Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

AMPK alpha 1 + AMPK alpha 2 was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab207442 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab207442 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : MCF7 whole cell lysate 10ug (Input).

Lane 2 : ab207442 IP in MCF7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207442 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 62 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins contains aa1-559 and aa1-552 with a His-Tag®.

All lanes:

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/2000 dilution

Lane 1:

Human AMPK alpha 1 full length recombinant protein at 0.01 µg

Lane 2:

Human AMPK alpha 2 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 62 kDa,64 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/10000 dilution

Lanes 3 - 4:

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 4:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 62 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 30 seconds; Lane 3-5 : 3 minutes Lane 6-9 : 5 seconds.

All lanes:

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Lane 5:

Rat spleen lysate at 10 µg

Lane 6:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 7:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 9:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 62 kDa

false

Carrier free

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19549

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed : 17307971, PubMed : 17712357, PubMed : 24563466, PubMed : 37821951). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed : 17307971, PubMed : 17712357). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed : 17307971, PubMed : 17712357). Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity). Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed : 34077757). Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity). Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed : 11518699, PubMed : 11554766, PubMed : 15866171, PubMed : 17711846, PubMed : 18184930). Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity). In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription (By similarity). Acts as a key regulator of cell growth and proliferation by phosphorylating FNIP1, TSC2, RPTOR, WDR24 and ATG1/ULK1 : in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed : 14651849, PubMed : 18439900, PubMed : 20160076, PubMed : 21205641). Also phosphorylates and inhibits GATOR2 subunit WDR24 in response to nutrient limitation, leading to suppress glucose-mediated mTORC1 activation (PubMed : 36732624). In response to energetic stress, phosphorylates FNIP1, inactivating the non-canonical mTORC1 signaling, thereby promoting nuclear translocation of TFEB and TFE3, and inducing transcription of lysosomal or autophagy genes (PubMed : 37079666). In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed : 21205641). In that process also activates WDR45/WIPI4 (PubMed : 28561066). Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed : 32029622). In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed : 17486097). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity). May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity). Also has tau-protein kinase activity : in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity). Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed : 12519745, PubMed : 20074060). Regulates hepatic lipogenesis. Activated via SIRT3, represses sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. Upon stress, regulates mitochondrial fragmentation through phosphorylation of MTFR1L (PubMed : 36367943).

See full target information PRKAA1

Additional targets

PRKAA2

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:537 PubMed41035048

2025

Linolamide in apoptotic bodies: a key factor in mesenchymal stem cell immunotherapy for two abortion models.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Shi,Xiaowen Lu,Haiyi Fei,Xiu Liu,Shiqian Xu,Yuhan Lin,Ling Fang,Lingling Jiang,Songying Zhang

Chinese medicine 20:112 PubMed40660305

2025

Acupuncture promotes muscle cells ATP metabolism in ST36 acupoint local exerting effect by activating TRPV1/CaMKII/AMPK/PGC1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihan Chen,Kaifang Yao,Xinrui Wang,Yangyang Liu,Simin Du,Shenjun Wang,Yuxin Fang,Yuan Xu,Zhifang Xu,Xiaowei Lin,Yi Guo

Genes & development 39:770-786 PubMed40324880

2025

Aneuploidy generates enhanced nucleotide dependency and sensitivity to metabolic perturbation.

Applications

Unspecified application

Species

Unspecified reactive species

Rayna Y Magesh,Arshia N Kaur,Faith N Keller,Abdulrazak Frederick,Tenzin Tseyang,John A Haley,Alejandra M Rivera-Nieves,Anthony C Liang,David A Guertin,Jessica B Spinelli,Stephen J Elledge,Emma V Watson

European journal of medical research 30:269 PubMed40211342

2025

Inhibition of esophageal squamous cell carcinoma progression by MIR210HG and activation of the P53 signaling pathway to promote apoptosis and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyu Wang,Zhenhu Zhang,Liang Song,Xiangyan Liu,Xiaopeng He

Poultry science 104:104967 PubMed40106851

2025

Fowl adenovirus serotype-4 induces autophagy via the AMPK-mTOR signaling pathway during viral invasion of host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongnuan Wang,Wei Li,Huixia Feng,Minrui Wu,Farwa Zainab,Jinen Wang,Chenyang Wang,Ding Zhang,Zilong Sun,Shijun J Zheng,Bo Yang

Open life sciences 20:20220971 PubMed40059880

2025

High-temperature requirement serine protease A2 inhibitor UCF-101 ameliorates damaged neurons in traumatic brain-injured rats by the AMPK/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Wen Qiu,Zhan Jin,Zhi-Zhan Fu,Xin-Jiang Yan,Cheng-Peng Zhan,Hui-Wen Zheng,Mei-Ying Li,Guo-Feng Yu

NPJ precision oncology 9:15 PubMed39809873

2025

A targetable OSGIN1 - AMPK - SLC2A3 axis controls the vulnerability of ovarian cancer to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Deng,Fan Tang,Xiangyu Chang,Yanqin Zhang,Penglin Liu,Xuechao Ji,Yubo Zhang,Ruiye Yang,Junyi Jiang,Junqi He,Jinwei Miao

Biotechnology and applied biochemistry 72:954-962 PubMed39722125

2024

PRKAA2 Promotes Tumor Growth and Inhibits Ferroptosis through SLC7A11/GSH/GPX4 Pathway in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Wei,Zhilian Zhou,Yu Zhang,Jie Wang,Ke Huang,Yuanyu Ding,Yingming Sun,Mingming Gu,Xiangang Kong,Erping Xi,Shaoshan Zeng

Inflammation 48:770-782 PubMed38954260

2024

Ginkgolide C attenuated Western diet-induced non-alcoholic fatty liver disease via increasing AMPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Xie,Leyi Wei,Jiashi Guo,Qingsong Jiang,Yang Xiang,Yan Lin,Huang Xie,Xinru Yin,Xia Gong,Jingyuan Wan

Scientific reports 14:12430 PubMed38816541

2024

Transgenic female mice producing trans 10, cis 12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Rao,Lu-Wen Liang,Mei-Juan Li,Yang-Yang Wang,Bao-Zhu Wang,Ke-Mian Gou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]

Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (50)

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Product promise