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Mouse Monoclonal AMPK alpha 1 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse, African green monkey, Recombinant fragment samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRKAA1.

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Images

Western blot - Anti-AMPK alpha 1 antibody [2B7] (AB110036), expandable thumbnail
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (AB110036), expandable thumbnail
  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (AB110036), expandable thumbnail
  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (AB110036), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (AB110036), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human PRKAA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13131

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISAWBIHC-PICC/IF
Human
Expected
Expected
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected
African green monkey
Expected
Expected
Tested
Expected
Expected
Recombinant fragment
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Rat

Dilution info

1/200.00000 - 1/1000.00000

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Human, Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/10000

Notes

-

Expected
Expected

Species

Rat, Human, Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Species

Mouse

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Species

African green monkey

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat, Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat, Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357, PubMed:24563466, PubMed:37821951). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357). Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity). Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed:34077757). Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity). Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed:11518699, PubMed:11554766, PubMed:15866171, PubMed:17711846, PubMed:18184930). Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity). In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription (By similarity). Acts as a key regulator of cell growth and proliferation by phosphorylating FNIP1, TSC2, RPTOR, WDR24 and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed:14651849, PubMed:18439900, PubMed:20160076, PubMed:21205641). Also phosphorylates and inhibits GATOR2 subunit WDR24 in response to nutrient limitation, leading to suppress glucose-mediated mTORC1 activation (PubMed:36732624). In response to energetic stress, phosphorylates FNIP1, inactivating the non-canonical mTORC1 signaling, thereby promoting nuclear translocation of TFEB and TFE3, and inducing transcription of lysosomal or autophagy genes (PubMed:37079666). In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed:21205641). In that process also activates WDR45/WIPI4 (PubMed:28561066). Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed:32029622). In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:17486097). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity). May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity). Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity). Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed:12519745, PubMed:20074060). Regulates hepatic lipogenesis. Activated via SIRT3, represses sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. Upon stress, regulates mitochondrial fragmentation through phosphorylation of MTFR1L (PubMed:36367943).

Alternative names

Recommended products

Mouse Monoclonal AMPK alpha 1 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse, African green monkey, Recombinant fragment samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRKAA1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human PRKAA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13131
Clone number

2B7

Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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7 product images

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1: Jurkat cell lysate.

    Lane 2: HeLa cell lysate.

    Lane 3: HepG2 cell lysate.

    Lane 4: MCF-7 cell lysate.

    Lane 5: Cos7 cell lysate.

    Lane 6: NIH3T3 cell lysate.

    Lane 7: K562 cell lysate.

    Lane 8: HEK293 cell lysate.

    Lane 9: PC-12 cell lysate.

    Predicted band size: 64 kDa

    Observed band size: 64 kDa

  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.

  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    The proposed dilution of ab110036 is 1/10000.

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    False colour image of Western blot: Anti-AMPK alpha 1 antibody [2B7] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110036 was shown to bind specifically to AMPK alpha 1. A band was observed at 64 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in PRKAA1 knockout cell line Mouse PRKAA1 knockout RAW 264.7 cell line ab280055 (knockout cell lysate Mouse PRKAA1 knockout RAW 264.7 cell lysate ab280114). To generate this image, wild-type and PRKAA1 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1: Wild-type RAW 264.7 cell lysate at 20 µg

    Lane 2: PRKAA1 knockout RAW 264.7 cell lysate at 20 µg

    Lane 3: Mouse Liver cell lysate at 20 µg

    Lane 4: Neuro2A cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 64 kDa

    Observed band size: 64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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