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Rabbit Recombinant Monoclonal AMPK alpha 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (AB210714), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (AB210714), expandable thumbnail
  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (AB210714), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (AB210714), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (AB210714), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

This antibody shows low sensitivity in mouse and rat samples.

Species
Mouse
Dilution info
-
Notes

This antibody shows low sensitivity in mouse and rat samples.

Species
Rat
Dilution info
-
Notes

This antibody shows low sensitivity in mouse and rat samples.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

8 products for Alternative Version

Target data

Function

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357, PubMed:24563466, PubMed:37821951). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357). Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity). Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed:34077757). Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity). Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed:11518699, PubMed:11554766, PubMed:15866171, PubMed:17711846, PubMed:18184930). Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity). In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription (By similarity). Acts as a key regulator of cell growth and proliferation by phosphorylating FNIP1, TSC2, RPTOR, WDR24 and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed:14651849, PubMed:18439900, PubMed:20160076, PubMed:21205641). Also phosphorylates and inhibits GATOR2 subunit WDR24 in response to nutrient limitation, leading to suppress glucose-mediated mTORC1 activation (PubMed:36732624). In response to energetic stress, phosphorylates FNIP1, inactivating the non-canonical mTORC1 signaling, thereby promoting nuclear translocation of TFEB and TFE3, and inducing transcription of lysosomal or autophagy genes (PubMed:37079666). In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed:21205641). In that process also activates WDR45/WIPI4 (PubMed:28561066). Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed:32029622). In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:17486097). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity). May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity). Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity). Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed:12519745, PubMed:20074060). Regulates hepatic lipogenesis. Activated via SIRT3, represses sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. Upon stress, regulates mitochondrial fragmentation through phosphorylation of MTFR1L (PubMed:36367943).

Alternative names

AMPK1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase

Recommended products

Rabbit Recombinant Monoclonal AMPK alpha 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
Y365
Purification technique
Affinity purification Protein A
Specificity

This antibody is specific for human AMPK alpha 1. This antibody shows low sensitivity in mouse and rat samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab210714 is the carrier-free version of Anti-AMPK alpha 1 antibody [Y365] ab32047.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    False colour image of Western blot: Anti-AMPK alpha 1 antibody [Y365] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-AMPK alpha 1 antibody [Y365] ab32047 was shown to bind specifically to AMPK alpha 1. A band was observed at 64 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in PRKAA1 knockout cell line Mouse PRKAA1 knockout RAW 264.7 cell line ab280055 (knockout cell lysate Mouse PRKAA1 knockout RAW 264.7 cell lysate ab280114). To generate this image, wild-type and PRKAA1 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye$®$ 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye$®$ 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047) at 1/1000 dilution

    Lane 1: Wild-type RAW 264.7 cell lysate at 20 µg

    Lane 2: PRKAA1 knockout RAW 264.7 cell lysate at 20 µg

    Lane 2: Western blot - Mouse PRKAA1 knockout RAW 264.7 cell line (Mouse PRKAA1 knockout RAW 264.7 cell line ab280055)

    Lane 3: Mouse Liver cell lysate at 20 µg

    Lane 4: Neuro2A cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 64 kDa

    Observed band size: 64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling AMPK alpha 1 with purified Anti-AMPK alpha 1 antibody [Y365] ab32047 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Anti-AMPK alpha 1 antibody [Y365] ab32047 (purified) at 1/40 immunoprecipitating AMPK alpha 1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10μg)

    Lane 2 (+): Anti-AMPK alpha 1 antibody [Y365] ab32047 + HeLa whole cell lysate (10μg).

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AMPK alpha 1 antibody [Y365] ab32047 in HeLa whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    All lanes: Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047)

    Predicted band size: 64 kDa

    Observed band size: 63 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling AMPK alpha 1 with purified Anti-AMPK alpha 1 antibody [Y365] ab32047 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Intracellular Flow Cytometry analysis of HeLa cells labelling AMPK alpha 1 with purified Anti-AMPK alpha 1 antibody [Y365] ab32047 at 1/150 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This antibody shows low sensitivity in mouse samples.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse retina tissue lysate at 20 µg

    Lane 7: Mouse skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa

    Observed band size: 40 kDa, 63 kDa

    Exposure time: 3min

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Blocking and dilution buffer: 5% NFDM/TBST.
    This antibody shows low sensitivity in mouse and rat samples.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047) at 1/2000 dilution

    Lane 1: C6 cell lysate at 20 µg

    Lane 2: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 64 kDa

    Observed band size: 63 kDa

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This antibody shows low sensitivity in mouse and rat samples.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    All lanes: Western blot - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047) at 1/20000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 6: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa

    Observed band size: 63 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Overlay histogram showing HeLa cells stained with unpurified Anti-AMPK alpha 1 antibody [Y365] ab32047 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified Anti-AMPK alpha 1 antibody [Y365] ab32047, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analaysis of human cervical carcinoma tissue labelling AMPK alpha 1 with unpurified Anti-AMPK alpha 1 antibody [Y365] ab32047 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714), expandable thumbnail

    Western blot - Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AMPK alpha 1 antibody [Y365] ab32047).

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/1000000 dilution.

    This blot was developed using a higher sensitivity ECL substrate.

    Compared with Anti-AMPK alpha 1 antibody [Y365] ab32047, Anti-AMPK alpha 1 antibody [EPR24413-70] ab271188 has higher sensitivity, we recommend Anti-AMPK alpha 1 antibody [EPR24413-70] ab271188 as an alternative for testing AMPK alpha 1 in mouse and rat samples in western blot.

    Lanes 1 - 8: Western blot - Anti-AMPK alpha 1 antibody [Y365] (Anti-AMPK alpha 1 antibody [Y365] ab32047) at 1/1000 dilution

    Lanes 1 - 8: Western blot - Anti-AMPK alpha 1 antibody [EPR24413-70] (Anti-AMPK alpha 1 antibody [EPR24413-70] ab271188) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse heart tissue lysate at 20 µg

    Lane 6: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 7: Rat brain tissue lysate at 20 µg

    Lane 8: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 64 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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