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Rabbit Recombinant Monoclonal AMPK alpha 1 phospho T183 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (AB271892), expandable thumbnail
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (AB271892), expandable thumbnail
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (AB271892), expandable thumbnail
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (AB271892), expandable thumbnail
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (AB271892), expandable thumbnail

Publications

  • Chemical biology & drug design 103:e144742024
    Hydromorphone hydrochloride preconditioning combined with postconditioning attenuates myocardial ischemia/reperfusion injury in rats by improving mitochondrial function and activating the PI3K/Akt signaling pathway.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Liuji Qiu,Yan Yan,Guocheng Zhong,Zhiqi Hou,Yongcai Ye,Jiaying Lin,Dexing Luo
    PubMed 38342769

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Not recommended
Tested
Mouse
Not recommended
Tested
Rat
Not recommended
Tested
Drosophila melanogaster
Not recommended
Tested

Not recommended
Not recommended

Species

Human, Mouse, Rat, Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat, Drosophila melanogaster, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In that process also activates WDR45 (PubMed:28561066). In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

      Alternative names

      5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase, PRKAA1, AMPK1

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      Rabbit Recombinant Monoclonal AMPK alpha 1 phospho T183 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 1 publication.

      Alternative names

      5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase, PRKAA1, AMPK1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR5683

      Purification technique

      Affinity purification Protein A

      Specificity

      This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

      Dissociation constant

      1.22 x 10-11 M

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab271892 is the carrier-free version of Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      7 product images

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        Blocking and dilution buffer: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/2000 dilution

        Lane 1: HEK-293 (human embryonic kidney) whole cell lysates at 10 µg

        Lane 2: HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides at 10 µg

        Lane 3: HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides at 10 µg

        Secondary

        All lanes: Goat anti-rabbit IgG, (H+L), HRP conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Observed band size: 64 kDa

        Exposure time: 15s

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000 dilution

        All lanes: Fruit fly lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

        Predicted band size: 97 kDa

        Observed band size: 97 kDa

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000 dilution

        Lane 1: Mouse heart lysate at 10 µg

        Lane 2: Rat brain lysate at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000 dilution

        All lanes: Molt-4 cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000 dilution

        Lane 1: Untreated HEK-293 cell lysate at 10 µg

        Lane 2: HEK-293 cell lysate treated with Lambda phosphatase at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - BSA and Azide free (ab271892)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

        All lanes: Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000 dilution

        Lane 1: 293 cell lysate at 10 µg

        Lane 2: Lysate of 293 cells treated with lambda phosphatase at 10 µg

        Secondary

        All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      • OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892), expandable thumbnail

        OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)

        We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
        Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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