AB271892

Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AMPK alpha 1 phospho T183 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 1 publication.

View Alternative Names

AMPK1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase

7 Images

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Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (<a href='/en-us/products/primary-antibodies/ampk-alpha-1-phospho-t183-ampk-alpha-2-phospho-t172-antibody-epr5683-ab133448'>ab133448</a>) at 1/1000 dilution

Lane 1:

293 cell lysate at 10 µg

Lane 2:

Lysate of 293 cells treated with lambda phosphatase at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

false

Unknown

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Lane 1:

Untreated HEK-293 cell lysate at 10 µg

Lane 2:

HEK-293 cell lysate treated with Lambda phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

false

Unknown

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Blocking and dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Lane 1:

HEK-293 (human embryonic kidney) whole cell lysates at 10 µg

Lane 2:

HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides at 10 µg

Lane 3:

HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), HRP conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 64 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Molt-4 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

false

Unknown

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

false

Unknown

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).

All lanes:

Fruit fly lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

OI-RD Scanning - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
Carrier free

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5683

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

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Reactivity data

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Product details

ab271892 is the carrier-free version of ab133448.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed : 17307971, PubMed : 17712357, PubMed : 24563466, PubMed : 37821951). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed : 17307971, PubMed : 17712357). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed : 17307971, PubMed : 17712357). Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity). Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed : 34077757). Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity). Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed : 11518699, PubMed : 11554766, PubMed : 15866171, PubMed : 17711846, PubMed : 18184930). Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity). In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription (By similarity). Acts as a key regulator of cell growth and proliferation by phosphorylating FNIP1, TSC2, RPTOR, WDR24 and ATG1/ULK1 : in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed : 14651849, PubMed : 18439900, PubMed : 20160076, PubMed : 21205641). Also phosphorylates and inhibits GATOR2 subunit WDR24 in response to nutrient limitation, leading to suppress glucose-mediated mTORC1 activation (PubMed : 36732624). In response to energetic stress, phosphorylates FNIP1, inactivating the non-canonical mTORC1 signaling, thereby promoting nuclear translocation of TFEB and TFE3, and inducing transcription of lysosomal or autophagy genes (PubMed : 37079666). In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed : 21205641). In that process also activates WDR45/WIPI4 (PubMed : 28561066). Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed : 32029622). In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed : 17486097). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity). May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity). Also has tau-protein kinase activity : in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity). Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed : 12519745, PubMed : 20074060). Regulates hepatic lipogenesis. Activated via SIRT3, represses sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. Upon stress, regulates mitochondrial fragmentation through phosphorylation of MTFR1L (PubMed : 36367943).

See full target information PRKAA1 phospho T183

Additional targets

PRKAA2 phospho T172

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 103:e14474 PubMed38342769

2024

Hydromorphone hydrochloride preconditioning combined with postconditioning attenuates myocardial ischemia/reperfusion injury in rats by improving mitochondrial function and activating the PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liuji Qiu,Yan Yan,Guocheng Zhong,Zhiqi Hou,Yongcai Ye,Jiaying Lin,Dexing Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

OI-RD Scanning - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683] - BSA and Azide free (AB271892)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (1)

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