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AB284711

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal AMPK beta 1 phospho S181 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

AMPK, PRKAB1, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb

3 Images
Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)
  • WB

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)

This data was developed using ab68206, the same antibody clone in a different buffer formulation.

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (<a href='/en-us/products/primary-antibodies/ampk-beta-1-phospho-s181-antibody-ep2181y-ab68206'>ab68206</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg with NFDM/TBST

Lane 2:

293T whole cell lysate, membrane incubated with alkaline phosphatase at 15 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 20s

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)
  • WB

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)

This data was developed using ab68206, the same antibody clone in a different buffer formulation.

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (<a href='/en-us/products/primary-antibodies/ampk-beta-1-phospho-s181-antibody-ep2181y-ab68206'>ab68206</a>) at 1/10000 dilution

Lane 1:

Rat heart lysate at 15 µg with NFDM/TBST

Lane 2:

Rat heart lysate, membrane incubated with alkaline phosphatase at 15 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)
  • WB

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - BSA and Azide free (AB284711)

This data was developed using ab68206, the same antibody clone in a different buffer formulation.

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (<a href='/en-us/products/primary-antibodies/ampk-beta-1-phospho-s181-antibody-ep2181y-ab68206'>ab68206</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg with NFDM/TBST

Lane 2:

Mouse brain lysate, membrane incubated with alkaline phosphatase at 15 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

  • Unconjugated

    Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2181Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284711 is the carrier-free version of ab68206.

This product has switched from a hybridoma to recombinant production method on 7th February 2025.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
See full target information PRKAB1 phospho S181
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Complementary Products

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Western blot - Anti-AMPK alpha 1 antibody [Y365] (AB32047)

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Primary Antibodies

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Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

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Secondary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com