JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB32382

Anti-AMPK gamma 1 antibody [Y307]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal AMPK gamma 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] (AB32382)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] (AB32382)

This image shows paraffin embedded human skeletal muscle stained with 1/250 ab23282.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)
  • WB

Unknown

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)

All lanes:

Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

HeLa cell lysate

Predicted band size: 38 kDa

Observed band size: 37 kDa

false

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)
  • WB

Lab

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)

Lanes 1 - 3 : Merged signal (red and green). Green - ab32382 observed at 38 kDa. Red - loading control, Mouse anti Vinculin, observed at 125 kDa.

ab32382 was shown to recognize AMPK gamma 1 in wild-type HAP1 cells as signal was lost at the expected MW in PRKAG1 (AMPK gamma 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PRKAG1 (AMPK gamma 1) knockout samples were subjected to SDS-PAGE. ab32382 and Mouse anti-Vinculin (ab9484 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PRKAG1 (AMPK gamma 1) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Predicted band size: 38 kDa

false

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)
  • WB

AbReview17601****

Western blot - Anti-AMPK gamma 1 antibody [Y307] (AB32382)

All lanes:

Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

Lane 1:

HeLa whole cell lystate - Control Si

Lane 2:

HeLa whole cell lystate - treated with AMPK gamma 1 siRNA A for 36 hours

Lane 3:

HeLa whole cell lystate - treated with AMPK gamma 1 siRNA B for 36 hours

Secondary

All lanes:

HRP-conjugated Donkey polyclonal to rabbit IgG at 1/3000 dilution

Predicted band size: 38 kDa

false

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y307

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab32382 reacts with an epitope located in the N terminal region of AMPK gamma 1.

style
left-column

Alternative Products

Primary Antibodies

AB239818

Anti-AMPK gamma 1 antibody [Y308] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] - BSA and Azide free (AB239818)

  • 0
  • 0
Primary Antibodies

AB223116

Anti-AMPK gamma 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AMPK gamma 1 antibody (AB223116)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133448

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (AB133448)

  • 4
  • 5
Primary Antibodies

AB207442

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (AB207442)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed : 21680840, PubMed : 24563466). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed : 21680840, PubMed : 24563466). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed : 21680840, PubMed : 24563466). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed : 21680840, PubMed : 24563466). Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK : AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits (PubMed : 21680840, PubMed : 24563466). ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit (PubMed : 21680840, PubMed : 24563466). ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive (PubMed : 21680840, PubMed : 24563466).
See full target information PRKAG1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:16 PubMed36681672

2023

Resveratrol protects osteocytes against oxidative stress in ovariectomized rats through AMPK/JNK1-dependent pathway leading to promotion of autophagy and inhibition of apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Wei,Shuang Chai,Chen Yue,Hong Zhang,Jitian Li,Na Qin

Autophagy 16:1871-1888 PubMed31983282

2020

DNA-dependent protein kinase regulates lysosomal AMP-dependent protein kinase activation and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Pietri Puustinen,Anne Keldsbo,Elisabeth Corcelle-Termeau,Kevin Ngoei,Stine L Sønder,Thomas Farkas,Klaus Kaae Andersen,Jon S Oakhill,Marja Jäättelä

PloS one 11:e0166740 PubMed27851811

2016

Atorvastatin Alleviates Experimental Diabetic Cardiomyopathy by Regulating the GSK-3β-PP2Ac-NF-κB Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Min Ren,Guang-Feng Zuo,Wen Wu,Jie Luo,Peng Ye,Shao-Liang Chen,Zuo-Ying Hu

Proceedings of the National Academy of Sciences of 112:E7276-85 PubMed26655341

2015

Antifungal drug itraconazole targets VDAC1 to modulate the AMPK/mTOR signaling axis in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Head,Wei Shi,Liang Zhao,Kirill Gorshkov,Kalyan Pasunooti,Yue Chen,Zhiyou Deng,Ruo-jing Li,Joong Sup Shim,Wenzhi Tan,Thomas Hartung,Jin Zhang,Yingming Zhao,Marco Colombini,Jun O Liu

International journal of cancer 135:2046-53 PubMed24676687

2014

Inactivation of protein-phosphatase 2A causing hyperphosphorylation of autoantigenic paraprotein targets in MGUS/MM is due to an exchange of its regulatory subunits.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus-Dieter Preuss,Natalie Fadle,Evi Regitz,Gerhard Held,Michael Pfreundschuh
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com