Rabbit Recombinant Monoclonal AMPK gamma 1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 24 publications.
Images
![Western blot - Anti-AMPK gamma 1 antibody [Y308] (AB32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--western-blot-img140123.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMPK gamma 1 antibody [Y308] (AB32508)")
![Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] (AB32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--flow-cytometry-intracellular-img24983.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] (AB32508)")
![Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (AB32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--immunoprecipitation-img20574.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (AB32508)")
![Western blot - Anti-AMPK gamma 1 antibody [Y308] (AB32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--western-blot-img29783.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AMPK gamma 1 antibody [Y308] (AB32508)")
Publications
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- The Journal of biological chemistry 298:1018522022Natural (dihydro)phenanthrene plant compounds are direct activators of AMPK through its allosteric drug and metabolite-binding site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Sanders,Yann Ratinaud,Katyayanee Neopane,Nicolas Bonhoure,Emily A Day,Olivier Ciclet,Steve Lassueur,Martine Naranjo Pinta,Maria Deak,Benjamin Brinon,Stefan Christen,Gregory R Steinberg,Denis Barron,Kei SakamotoPubMed 35331736
- Aging 13:16834-168582021The consequences of a high-calorie diet background before calorie restriction on skeletal muscles in a mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Maldonado,Jianying Chen,Yang Lujun,Huiqin Duan,Mazhar Ali Raja,Ting Qu,Tianhua Huang,Jiang Gu,Ying ZhongPubMed 34166224
- Pharmaceuticals (Basel, Switzerland) 14:2021Regulation of AMPK Activity by CRBN Is Independent of the Thalidomide-CRL4 Protein Degradation Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung-Joo Yang,Seungje Jeon,Jeong Won Baek,Kwang Min Lee,Chul-Seung ParkPubMed 34073624
- Frontiers in pharmacology 12:6302102021Jian-Pi-Yi-Shen Formula Ameliorates Oxidative Stress, Inflammation, and Apoptosis by Activating the Nrf2 Signaling in 5/6 Nephrectomized Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanyuan Zhou,Xiaohu Zou,Jing Zhang,Ziwei Wang,Yajun Yang,Dongtao WangPubMed 33841151
- Molecular metabolism :1011832021Deletion of intestinal epithelial AMP-activated protein kinase alters distal colon permeability but not glucose homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSéverine Olivier et. al.PubMed 33548500
- Molecular therapy : the journal of the American So 28:1133-11532020AMPK Complex Activation Promotes Sarcolemmal Repair in Dysferlinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroya Ono et. al.PubMed 32087766
- Cell death & disease 11:1152020SIRT1 inhibits chemoresistance and cancer stemness of gastric cancer by initiating an AMPK/FOXO3 positive feedback loop.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei An et. al.PubMed 32051395
- Autophagy 16:1871-18882020DNA-dependent protein kinase regulates lysosomal AMP-dependent protein kinase activation and autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPietri Puustinen,Anne Keldsbo,Elisabeth Corcelle-Termeau,Kevin Ngoei,Stine L Sønder,Thomas Farkas,Klaus Kaae Andersen,Jon S Oakhill,Marja JäätteläPubMed 31983282
- Cell reports 29:1511-1523.e52019Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Hongying An,Ting Liu,Caolitao Qin,Hiromi Sesaki,Shaodong Guo,Sally Radovick,Mehboob Hussain,Akhil Maheshwari,Fredric E Wondisford,Brian O'Rourke,Ling HePubMed 31693892
- Journal of cellular physiology 235:1129-11402019SCD1 regulates the AMPK/SIRT1 pathway and histone acetylation through changes in adenine nucleotide metabolism in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Dziewulska,Aneta M Dobosz,Agnieszka Dobrzyn,Agnieszka Smolinska,Katarzyna Kolczynska,James M Ntambi,Pawel DobrzynPubMed 31241768
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | IHC | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Expected | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Expected | Predicted |
| African green monkey | Not recommended | Predicted | Not recommended | Predicted | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, African green monkey | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, African green monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey | Dilution info - | Notes - |
Target data
Function
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:21680840, PubMed:24563466). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:21680840, PubMed:24563466). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:21680840, PubMed:24563466). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:21680840, PubMed:24563466). Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits (PubMed:21680840, PubMed:24563466). ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit (PubMed:21680840, PubMed:24563466). ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive (PubMed:21680840, PubMed:24563466).
Alternative names
5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1
Recommended products
Rabbit Recombinant Monoclonal AMPK gamma 1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 24 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Y308
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognises 5'-AMP-activated protein kinase (AMPK).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--western-blot-img140123.jpg "Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
All lanes: Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution
All lanes: HEK293 whole cell lysate at 30 µg
SecondaryAll lanes: Alexa Fluor® 690-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 5min
![Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] (ab32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--flow-cytometry-intracellular-img24983.jpg "Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] (ab32508)")
Flow Cytometry (Intracellular) - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling AMPK gamma 1 with ab32508 at 1/100 dilution (1μg) (Red). Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
![Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--immunoprecipitation-img20574.jpg "Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508)")
Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
AMPK gamma 1 was immunoprecipitated using 1 mg Jurkat whole cell extract, 0.2 ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50μl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697). Bands: 37kDa: AMPK gamma 1.
All lanes: Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
Predicted band size: 38 kDa
![Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508), expandable thumbnail](https://content.abcam.com/products/images/ampk-gamma-1-antibody-y308-ab32508--western-blot-img29783.jpg "Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)")
Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
All lanes: Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution
All lanes: Jurkat cell lysate
Predicted band size: 38 kDa
Observed band size: 38 kDa
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