AB249171

Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal AMPS antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

AMPS, ADSL, Adenylosuccinate lyase, ASL, Adenylosuccinase, ASase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

This data was developed using ab154872, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling AMPS with ab154872 at 1/250 dilution.

Unknown

Western blot - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

This data was developed using ab154872, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AMPS antibody [EPR10746(B)] (<a href='/en-us/products/primary-antibodies/amps-antibody-epr10746b-ab154872'>ab154872</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw 264.7 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Lane 6:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 55 kDa

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Supplier Data

Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

This data was developed using ab154872, the same antibody clone in a different buffer formulation.

AMPS was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell extract, 10 μg (Input).

Lane 2 : ab154872 IP in NIH/3T3 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] (<a href='/en-us/products/primary-antibodies/amps-antibody-epr10746b-ab154872'>ab154872</a>)

Predicted band size: 55 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10746(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249171 is the carrier-free version of ab154872.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes two non-sequential steps in de novo AMP synthesis : converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.

See full target information ADSL

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

Western blot - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise