AB249082

Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AMPS antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AMPS, ADSL, Adenylosuccinate lyase, ASL, Adenylosuccinase, ASase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154182 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154182 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells with ab154182 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue labeling AMPS with ab154182 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154182 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154182 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

This data was developed using ab154182, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AMPS antibody [EPR10747(B)] (<a href='/en-us/products/primary-antibodies/amps-antibody-epr10747b-ab154182'>ab154182</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HCT-116 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa

false

Unconjugated

Anti-AMPS antibody [EPR10747(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10747(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249082 is the carrier-free version of ab154182.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes two non-sequential steps in de novo AMP synthesis : converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.

See full target information ADSL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Flow Cytometry (Intracellular) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Western blot - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (AB249082)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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