Anti-AMSH antibody [EPR4361] - BSA and Azide free

• WB

Lab

Western blot - Anti-AMSH antibody [EPR4361] - BSA and Azide free (AB247622)

This data was developed using the same antibody clone in a different buffer formulation (ab108301).

Lanes 1-4 : Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.

ab108301 Anti-STAMBP antibody was shown to specifically react with STAM Binding Protein in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265249 (knockout cell lysate ab258213) was used. Wild-type and STAM Binding Protein knockout samples were subjected to SDS-PAGE. ab108301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AMSH antibody [EPR4361] (<a href='/en-us/products/primary-antibodies/amsh-antibody-epr4361-ab108301'>ab108301</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

STAM Binding Protein knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human STAMBP (AMSH) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-stambp-amsh-knockout-hela-cell-line-ab265249'>ab265249</a>)

Lane 3:

HAP1 lysate at 20 µg

Lane 4:

Jurkat lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa

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