AB103580

Anti-Amylin/DAP antibody

Name: Anti-Amylin/DAP antibody (ab103580) | Abcam
Brand: Abcam Limited
SKU: AB103580

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(4 Publications)

Rabbit Polyclonal Amylin/DAP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Islet amyloid polypeptide.

View Alternative Names

Islet amyloid polypeptide, IAPP, Amylin, Diabetes-associated peptide, Insulinoma amyloid peptide, DAP

1 Images

Unknown

Western blot - Anti-Amylin/DAP antibody (AB103580)

All lanes:

Western blot - Anti-Amylin/DAP antibody (ab103580) at 1/500 dilution

All lanes:

Human spleen tissue lysate at 50 µg

Predicted band size: 10 kDa,11 kDa,12 kDa,13 kDa,14 kDa,15 kDa,18 kDa,6 kDa,8 kDa

Observed band size: 10 kDa,11 kDa,12 kDa,15 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Islet amyloid polypeptide.

P10997

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Amylin also known as Islet Amyloid Polypeptide (IAPP) is a peptide hormone mainly produced and co-secreted with insulin by pancreatic beta cells. This peptide has a molecular mass of approximately 3.9 kDa. Amylin is highly expressed in the pancreas with some expression in the gastrointestinal tract and the central nervous system. In images DAP typically refers to 4'6-diamidino-2-phenylindole which isn't directly related to amylin but this clarification can avoid confusion in laboratory discussions.

Biological function summary

Amylin peptide regulates blood glucose levels by slowing gastric emptying and promoting satiety. It acts as part of a complex with calcitonin receptor and a receptor activity-modifying protein to mediate its effects efficiently. Amylin influences the central nervous system by affecting appetite and has roles in bone metabolism through interactions with receptors related to alkaline phosphatase 138.

Pathways

Amylin functions in the glucose homeostasis and energy regulation pathways. It closely works with insulin impacting how the body manages postprandial glucose spikes. The peptide also interacts with cAMP signaling which is part of the same network that involves proteins like glucagon-like peptide-1 (GLP-1) which harmonizes action between different metabolic regulatory components.

Amylin accumulates in the form of amyloid deposits in type 2 diabetes contributing to the degeneration of beta cells. The presence of amylin aggregates connects with insulin in this pathology aggravating disease progression. Moreover the peptide plays a part in Alzheimer's disease where its amyloidogenic properties may have overlapping mechanisms with amyloid beta deposits impacting neurodegeneration through similar receptor pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Amylin/IAPP is a glucoregulatory peptide hormone that plays an important role in the regulation of energy homeostasis (PubMed : 2690069). Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. IAPP function is mediated by the CALCR-RAMPs (AMYRs) receptor complexes (By similarity). Amylin can also bind CALCR receptor in the absence of RAMPs, although it is more selective for AMYRs (By similarity).

See full target information Islet amyloid polypeptide

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 14: PubMed38398700

2024

Construction and Identification of a Breast Bioreactor for Human-Derived Hypoglycemic Protein Amylin.

Applications

Unspecified application

Species

Unspecified reactive species

Kongwei Huang,Xiuying Yan,Zhipeng Li,Fuhang Liu,Kuiqing Cui,Qingyou Liu

Cancers 14: PubMed36077845

2022

Pramlintide: A Novel Therapeutic Approach for Osteosarcoma through Metabolic Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzheng Yang,Zhanglong Peng,Elsa R Flores,Eugenie S Kleinerman

Biochimica et biophysica acta. Molecular cell research 1868:118971 PubMed33515645

2021

Human amylin aggregates release within exosomes as a protective mechanism in pancreatic β cells: Pancreatic β-hippocampal cell communication.

Applications

Unspecified application

Species

Unspecified reactive species

J Burillo,M Fernández-Rhodes,M Piquero,P López-Alvarado,J C Menéndez,B Jiménez,C González-Blanco,P Marqués,C Guillén,M Benito

Nature 517:626-30 PubMed25409149

2014

IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo.

Applications

Species

Unspecified reactive species

Avinashnarayan Venkatanarayan,Payal Raulji,William Norton,Deepavali Chakravarti,Cristian Coarfa,Xiaohua Su,Santosh K Sandur,Marc S Ramirez,Jaehuk Lee,Charles V Kingsley,Eliot F Sananikone,Kimal Rajapakshe,Katherine Naff,Jan Parker-Thornburg,James A Bankson,Kenneth Y Tsai,Preethi H Gunaratne,Elsa R Flores

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Amylin/DAP antibody

Western blot - Anti-Amylin/DAP antibody (AB103580)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise