AB55411

Anti-Amylin/DAP antibody

Name: Anti-Amylin/DAP antibody (ab55411) | Abcam
Brand: Abcam Limited
SKU: AB55411

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(3 Publications)

Rabbit Polyclonal Amylin/DAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Islet amyloid polypeptide.

View Alternative Names

Islet amyloid polypeptide, IAPP, Amylin, Diabetes-associated peptide, Insulinoma amyloid peptide, DAP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody (AB55411)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody (AB55411)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.

Supplier Data

Western blot - Anti-Amylin/DAP antibody (AB55411)

HeLa cell lysate

15ug per lane

5% milk for blocking

All lanes:

Western blot - Anti-Amylin/DAP antibody (ab55411)

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Islet amyloid polypeptide. The exact immunogen used to generate this antibody is proprietary information.

P10997

Specificity

ab55411 is expected to detect only the pro-form of Amylin/DAP, not the mature form after removal of the pro-peptide, amino acids 23-31.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Amylin also known as Islet Amyloid Polypeptide (IAPP) is a peptide hormone mainly produced and co-secreted with insulin by pancreatic beta cells. This peptide has a molecular mass of approximately 3.9 kDa. Amylin is highly expressed in the pancreas with some expression in the gastrointestinal tract and the central nervous system. In images DAP typically refers to 4'6-diamidino-2-phenylindole which isn't directly related to amylin but this clarification can avoid confusion in laboratory discussions.

Biological function summary

Amylin peptide regulates blood glucose levels by slowing gastric emptying and promoting satiety. It acts as part of a complex with calcitonin receptor and a receptor activity-modifying protein to mediate its effects efficiently. Amylin influences the central nervous system by affecting appetite and has roles in bone metabolism through interactions with receptors related to alkaline phosphatase 138.

Pathways

Amylin functions in the glucose homeostasis and energy regulation pathways. It closely works with insulin impacting how the body manages postprandial glucose spikes. The peptide also interacts with cAMP signaling which is part of the same network that involves proteins like glucagon-like peptide-1 (GLP-1) which harmonizes action between different metabolic regulatory components.

Amylin accumulates in the form of amyloid deposits in type 2 diabetes contributing to the degeneration of beta cells. The presence of amylin aggregates connects with insulin in this pathology aggravating disease progression. Moreover the peptide plays a part in Alzheimer's disease where its amyloidogenic properties may have overlapping mechanisms with amyloid beta deposits impacting neurodegeneration through similar receptor pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Amylin/IAPP is a glucoregulatory peptide hormone that plays an important role in the regulation of energy homeostasis (PubMed : 2690069). Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. IAPP function is mediated by the CALCR-RAMPs (AMYRs) receptor complexes (By similarity). Amylin can also bind CALCR receptor in the absence of RAMPs, although it is more selective for AMYRs (By similarity).

See full target information Islet amyloid polypeptide

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:24729 PubMed39433955

2024

The overexpression of human amylin in pancreatic β cells facilitate the appearance of amylin aggregates in the kidney contributing to diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

S Iglesias-Fortes,C González-Blanco,A García-Carrasco,A Izquierdo-Lahuerta,G García,A García-Aguilar,A Lockwood,O Palomino,G Medina-Gómez,M Benito,C Guillén

Journal of the neurological sciences 422:117315 PubMed33503519

2021

Detection of varicella zoster virus antigen and DNA in two cases of cerebral amyloid angiopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Mescher,Philip J Boyer,Andrew N Bubak,James E Hassell,Maria A Nagel

Reproduction (Cambridge, England) 150:449-62 PubMed26248480

2015

Isolation and characterisation of a novel trophoblast side-population from first trimester placentae.

Applications

Unspecified application

Species

Unspecified reactive species

J L James,D G Hurley,T K J B Gamage,T Zhang,R Vather,P Pantham,P Murthi,L W Chamley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Amylin/DAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody (AB55411)

Western blot - Anti-Amylin/DAP antibody (AB55411)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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