AB272414

Anti-Amylin/DAP antibody [EPR22555-52] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Amylin/DAP antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.

View Alternative Names

Islet amyloid polypeptide, IAPP, Amylin, Diabetes-associated peptide, Insulinoma amyloid peptide, DAP

1 Images

Sandwich ELISA - Anti-Amylin/DAP antibody [EPR22555-52] - BSA and Azide free (Capture) (AB272414)

sELISA

Supplier Data

Sandwich ELISA - Anti-Amylin/DAP antibody [EPR22555-52] - BSA and Azide free (Capture) (AB272414)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272208).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22555-52

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

style

left-column

Product details

ab272414 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Amylin. The recommended pair for sandwich ELISA is:
Capture: ab272414, Mouse Amylin Capture Antibody (unconjugated)
Detector: ab272415, Mouse Amylin Detector Antibody (unconjugated)
The reference range value is 526.749 - 6000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Amylin also known as Islet Amyloid Polypeptide (IAPP) is a peptide hormone mainly produced and co-secreted with insulin by pancreatic beta cells. This peptide has a molecular mass of approximately 3.9 kDa. Amylin is highly expressed in the pancreas with some expression in the gastrointestinal tract and the central nervous system. In images DAP typically refers to 4'6-diamidino-2-phenylindole which isn't directly related to amylin but this clarification can avoid confusion in laboratory discussions.

Biological function summary

Amylin peptide regulates blood glucose levels by slowing gastric emptying and promoting satiety. It acts as part of a complex with calcitonin receptor and a receptor activity-modifying protein to mediate its effects efficiently. Amylin influences the central nervous system by affecting appetite and has roles in bone metabolism through interactions with receptors related to alkaline phosphatase 138.

Pathways

Amylin functions in the glucose homeostasis and energy regulation pathways. It closely works with insulin impacting how the body manages postprandial glucose spikes. The peptide also interacts with cAMP signaling which is part of the same network that involves proteins like glucagon-like peptide-1 (GLP-1) which harmonizes action between different metabolic regulatory components.

Amylin accumulates in the form of amyloid deposits in type 2 diabetes contributing to the degeneration of beta cells. The presence of amylin aggregates connects with insulin in this pathology aggravating disease progression. Moreover the peptide plays a part in Alzheimer's disease where its amyloidogenic properties may have overlapping mechanisms with amyloid beta deposits impacting neurodegeneration through similar receptor pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Amylin/IAPP is a glucoregulatory peptide hormone that plays an important role in the regulation of energy homeostasis (PubMed : 2690069). Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. IAPP function is mediated by the CALCR-RAMPs (AMYRs) receptor complexes (By similarity). Amylin can also bind CALCR receptor in the absence of RAMPs, although it is more selective for AMYRs (By similarity).

See full target information Islet amyloid polypeptide

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Amylin/DAP antibody [EPR22555-52] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Amylin/DAP antibody [EPR22555-52] - BSA and Azide free (Capture) (AB272414)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise