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AB151216

Anti-ANAPC13 antibody

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(2 Publications)

Rabbit Polyclonal ANAPC13 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANAPC13 aa 1 to C-terminus.

View Alternative Names

Anaphase-promoting complex subunit 13, APC13, Cyclosome subunit 13, ANAPC13

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANAPC13 antibody (AB151216)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANAPC13 antibody (AB151216)

Immunohistochemical analysis of Paraffin embedded Human stomach tissue labeling ANAPC13 with ab151216 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ANAPC13 antibody (AB151216)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANAPC13 antibody (AB151216)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab151216 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-ANAPC13 antibody (AB151216)
  • WB

Unknown

Western blot - Anti-ANAPC13 antibody (AB151216)

All lanes:

Western blot - Anti-ANAPC13 antibody (ab151216) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

ANAPC13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Predicted band size: 8 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANAPC13 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BS18

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 15060174, PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 15060174, PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information ANAPC13
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed39861799

2025

Exploration of the Role of Cyclophilins in Established Hepatitis B and C Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Molle,Sarah Duponchel,Jennifer Rieusset,Michel Ovize,Alexander V Ivanov,Fabien Zoulim,Birke Bartosch

Experimental and therapeutic medicine 14:1487-1490 PubMed28810614

2017

Evolution of correlation between infection and autoimmune liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Guo Peng,Yu-Yuan Li,Hui-Ting Chen,Yan Zhou,Jian-Guo Ma,Hong-Min Yin
View all publications
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Product promise

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