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AB101984

Anti-Ancient ubiquitous protein 1 antibody

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(2 Publications)

Rabbit Polyclonal Ancient ubiquitous protein 1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AUP1 aa 350-450.

View Alternative Names

Lipid droplet-regulating VLDL assembly factor AUP1, Ancient ubiquitous protein 1, AUP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ancient ubiquitous protein 1 antibody (AB101984)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ancient ubiquitous protein 1 antibody (AB101984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Ancient ubiquitous protein 1 with ab101984 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-Ancient ubiquitous protein 1 antibody (AB101984)
  • IP

Unknown

Immunoprecipitation - Anti-Ancient ubiquitous protein 1 antibody (AB101984)

Detection of Ancient ubiquitous protein 1 by Western Blot of Immunprecipitate.
Lane 1 : ab101984, at 1 µg/ml, staining Ancient ubiquitous protein 1 in HeLa whole cell lysate immunoprecipitated using ab101984 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ancient ubiquitous protein 1 antibody (ab101984)

Predicted band size: 53 kDa

false

Western blot - Anti-Ancient ubiquitous protein 1 antibody (AB101984)
  • WB

Unknown

Western blot - Anti-Ancient ubiquitous protein 1 antibody (AB101984)

All lanes:

Western blot - Anti-Ancient ubiquitous protein 1 antibody (ab101984) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 53 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human AUP1 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q9Y679

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ancient ubiquitous protein 1 also known as AUP1 is a multifunctional protein with a mass of approximately 43 kDa. It plays an important role mechanically in the regulation of lipid droplet dynamics and protein degradation. AUP1 is ubiquitously expressed across various tissues with high levels observed in the liver and adipose tissue. It has been linked to cellular processes that manage protein quality control via ubiquitin-proteasome system.
Biological function summary

The Ancient ubiquitous protein 1 contributes to maintaining cellular homeostasis through its involvement in lipid metabolism and endoplasmic reticulum (ER) stress response. It acts as part of protein complexes that modulate ubiquitination activity influencing the fate of damaged or misfolded proteins. AUP1 interacts with various cellular machinery to stabilize lipid droplets impacting energy storage and usage. By engaging with these complexes it safeguards cells under stress conditions supporting proper cellular function.

Pathways

Ancient ubiquitous protein 1 influences both the ubiquitin-proteasome pathway and ER-associated degradation pathway. It collaborates with ubiquitin-related proteins like ubiquitin-conjugating enzyme UBE2G2 to enhance protein turnover maintaining protein homeostasis and stress resilience. By participating in these pathways AUP1 enables proper protein folding and disposal which are essential for cellular adaptation and response to metabolic demands and stress signals.

Ancient ubiquitous protein 1 has associations with metabolic disorders and neurodegenerative diseases. Dysregulation of AUP1 can lead to liver disease and type 2 diabetes due to its role in lipid storage and ER stress. Additionally AUP1 interactions with proteins like sequestosome 1 link it to neurodegenerative conditions where disrupted protein degradation and cellular stress responses contribute to disease progression. Understanding its function in these contexts can illuminate therapeutic approaches for these complex disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome (PubMed : 18711132, PubMed : 21857022). Plays a role in lipid droplet formation (PubMed : 21857022). Induces lipid droplet clustering (PubMed : 24039768). Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation (PubMed : 21127063, PubMed : 23223569). Also required for the degradation of INSIG1, SREBF1 and SREBF2 (PubMed : 23223569). Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB (PubMed : 28183703).. (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles.
See full target information AUP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2698 PubMed37164963

2023

Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Lan,Sophie Wilhelmina van Leur,Julia Ayano Fernando,Ho Him Wong,Martin Kampmann,Lewis Siu,Jingshu Zhang,Mingyuan Li,John M Nicholls,Sumana Sanyal

Oncology letters 19:671-680 PubMed31897183

2020

POLR1B is upregulated and promotes cell proliferation in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Haitao Liu,Junjie Zhao,Xingjie Ma,Weibo Qi
View all publications
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Product promise

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