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Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody that is used to detect Androgen Receptor in Flow Cytometry, IHC-P. Suitable for Human samples.



- Over 50 publications

- Trusted since 2002


Images

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474), expandable thumbnail
  • Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3 - 7.5
Preservative: 0.09% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human AR aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P10275

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt
Human
Tested
Tested
Macaque monkey
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/25 - 1/50
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Macaque monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Macaque monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

The protein expressed by the AR gene functions as a steroid hormone receptor, a type of ligand-activated transcription factor that regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Its transcription factor activity can be modulated by coactivator and corepressor proteins, such as ZBTB7A, which recruits NCOR1 and NCOR2 to androgen response elements on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. The transcription activation can also be down-regulated by NR0B2. The protein is activated, though not phosphorylated, by HIPK3 and ZIPK/DAPK3. Notably, isoforms 3 and 4 of this protein lack the C-terminal ligand-binding domain and may thus constitutively activate transcription of specific gene sets independently of steroid hormones. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Recommended products

Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody that is used to detect Androgen Receptor in Flow Cytometry, IHC-P. Suitable for Human samples.



- Over 50 publications

- Trusted since 2002

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human AR aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P10275
Clone number
AR 441
Purification technique
Affinity purification Protein G
Specificity

This antibody is specific to a protein of 110 kD, identified as androgen receptor.
This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor.
This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.

Light chain type
unknown
Concentration
Loading...
Purification notes

purified immunoglobulin fraction.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), in Human samples.

Recommended positive controls


Prostate Carcinoma.

Trusted by the scientific community


Anti-Androgen Receptor [AR 441] (ab9474) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists


Anti-Androgen Receptor [AR 441] (ab9474) has over 10 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.

Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

Associated diseases and disorders

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474), expandable thumbnail

    Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)

    Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474), expandable thumbnail

    Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)

    Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (ab9474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (ab9474)

    ab9474 staining human prostate by IHC-P.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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