AB9474

Anti-Androgen Receptor antibody [AR 441]

Name: Anti-Androgen Receptor antibody [AR 441] (ab9474) | Abcam
Brand: Abcam Limited
SKU: AB9474
Price: 400 USD
Availability: InStock
Rating: 4 (12 reviews)

Lab Essentials
What is this?

(12 Reviews)

(57 Publications)

Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody detecting Androgen Receptor in Flow Cytometry, IHC-P. Suitable for Human.

- Over 50 publications
- Trusted since 2002

View Alternative Names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

ab9474 staining human prostate by IHC-P.

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Flow Cyt

Unknown

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Flow Cyt

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Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AR 441

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human AR aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P10275

Specificity

This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Androgen Receptor [AR 441] (ab9474) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Androgen Receptor [AR 441] (ab9474) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

purified immunoglobulin fraction.

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.

Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the AR gene functions as a steroid hormone receptor, a type of ligand-activated transcription factor that regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Its transcription factor activity can be modulated by coactivator and corepressor proteins, such as ZBTB7A, which recruits NCOR1 and NCOR2 to androgen response elements on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. The transcription activation can also be down-regulated by NR0B2. The protein is activated, though not phosphorylated, by HIPK3 and ZIPK/DAPK3. Notably, isoforms 3 and 4 of this protein lack the C-terminal ligand-binding domain and may thus constitutively activate transcription of specific gene sets independently of steroid hormones. This supplementary information is collated from multiple sources and compiled automatically.

See full target information AR

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Acta dermato-venereologica 105:adv43088 PubMed40753487

2025

High-content Image-based Drug Testing of Patients' Primary Fibroblasts Reveals Potential New Treatment Options for Localized Scleroderma.

Applications

Unspecified application

Species

Unspecified reactive species

Katariina Mähönen,Isabel Mogollon,Antti Hassinen,Minttu Polso,Katja Välimäki,Swapnil Potdar,Al-Amin Chowdhury,Annamari Ranki,Jani Saarela,Vilja Pietiäinen,Jaana Panelius

Cancer immunology, immunotherapy : CII 74:268 PubMed40650767

2025

TGF-β3 promotes vascular normalization of prostate cancer to potentiate immunotherapy and chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qiliang Teng,Niu Wang,Hanqi Lei,Tongyu Tong,Yupeng Guan,Mengjun Huang,Fei Cao,Bin Xu,Jia Yang,Yimian Huo,Wenping Chen,Ran Bi,Xuanqi Wang,Zhenyu Wang,Fu-Ying Tian,Bo Zhao,Jun Pang

International journal of molecular sciences 25: PubMed39456756

2024

Myo-Inositol and D-Chiro-Inositol Reduce DHT-Stimulated Changes in the Steroidogenic Activity of Adult Granulosa Cell Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Maria Wojciechowska,Paulina Zając,Justyna Gogola-Mruk,Magdalena Karolina Kowalik,Anna Ptak

Frontiers in endocrinology 15:1368494 PubMed38745948

2024

Homeobox regulator Wilms Tumour 1 is displaced by androgen receptor at cis-regulatory elements in the endometrium of PCOS patients.

Applications

Unspecified application

Species

Unspecified reactive species

David W James,Marcos Quintela,Lisa Lucini,Noor K Alkafri,Gareth D Healey,Kinza Younas,Adnan Bunkheila,Lavinia Margarit,Lewis W Francis,Deyarina Gonzalez,R Steven Conlan

International journal of molecular sciences 25: PubMed38542313

2024

Overexpression of REST Represses the Epithelial-Mesenchymal Transition Process and Decreases the Aggressiveness of Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastián Indo,Octavio Orellana-Serradell,María José Torres,Enrique A Castellón,Héctor R Contreras

Clinical case reports 11:e7310 PubMed37215961

2023

A rare case of a toddler with unilateral cryptorchidism-related cranial suspensory ligament remnant.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Nakajima,Kazuto Suda,Atsushi Arakawa,Toshihiro Yanai

Scientific reports 13:4683 PubMed36949059

2023

CACNA1D overexpression and voltage-gated calcium channels in prostate cancer during androgen deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Niamh McKerr,Adone Mohd-Sarip,Hannah Dorrian,Conor Breen,Jacqueline A James,Stephen McQuaid,Ian G Mills,Karen D McCloskey

Cell death & disease 13:927 PubMed36335093

2022

Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanrong Chen,Yi Shao,Wanqing Wei,Shimiao Zhu,Yang Li,Yutong Chen,Hanling Li,Hao Tian,Guijiang Sun,Yuanjie Niu,Zhiqun Shang

The Biochemical journal 479:1519-1531 PubMed35781489

2022

Identification of ELK1 interacting peptide segments in the androgen receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Soave,Charles Ducker,Seongho Kim,Thomas Strahl,Rayna Rosati,Yanfang Huang,Peter E Shaw,Manohar Ratnam

Molecular cancer research : MCR 20:841-853 PubMed35302608

2022

Attenuating Adaptive VEGF-A and IL8 Signaling Restores Durable Tumor Control in AR Antagonist-Treated Prostate Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela J Maxwell,Melanie McKechnie,Christopher W Armstrong,Judith M Manley,Chee Wee Ong,Jenny Worthington,Ian G Mills,Daniel B Longley,James P Quigley,Amina Zoubeidi,Johann S de Bono,Elena Deryugina,Melissa J LaBonte,David J J Waugh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Androgen Receptor antibody [AR 441]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (57)

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