Name: Anti-Androgen Receptor antibody [EPR1535(2)]
SKU: ab133273
Rating: 5 (11 reviews)

Anti-AR (androgen receptor) antibody [EPR1535(2)] is a rabbit monoclonal antibody that is used to detect AR in western blot, IHC and ICC/IF. Suitable for human, mouse and rat samples.

- Cited in over 120 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR1535(2) is cited in over 160 publications

Related conjugates and formulations

ab275124
Conjugated
Alexa Fluor® 555 Anti-Androgen Receptor antibody [EPR1535(2)]
ab194194
Conjugated
Alexa Fluor® 488 Anti-Androgen Receptor antibody [EPR1535(2)]
ab194196
Conjugated
HRP Anti-Androgen Receptor antibody [EPR1535(2)]
ab194195
Conjugated
Alexa Fluor® 647 Anti-Androgen Receptor antibody [EPR1535(2)]
ab271891
Carrier free
Anti-Androgen Receptor antibody [EPR1535(2)] - BSA and Azide free

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Expected
Rat	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes For unpurified use at 1/100-1/250 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes For unpurified use at 1/100-1/250 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes For unpurified use at 1/100-1/250 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes For unpurified use at 1:1000 For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Species Rat	Dilution info 1/2000	Notes For unpurified use at 1:1000 For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Species Human	Dilution info 1/2000	Notes For unpurified use at 1:1000 For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/250.00000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information AR

Function

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Alternative names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR1535(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has been cited over 124 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has 11 independent reviews from customers.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) specifically detects Androgen Receptor (UniProt ID: P10275; Molecular weight: 99kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR1535(2) - Anti-Androgen Receptor antibody [EPR1535(2)] - BSA and Azide free ab271891.

Antibody clone EPR1535(2) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, Alexa Fluor® 555 (Alexa Fluor® 488 Anti-Androgen Receptor antibody [EPR1535(2)] ab194194, Alexa Fluor® 647 Anti-Androgen Receptor antibody [EPR1535(2)] ab194195, HRP Anti-Androgen Receptor antibody [EPR1535(2)] ab194196, Alexa Fluor® 555 Anti-Androgen Receptor antibody [EPR1535(2)] ab275124).

The androgen receptor (AR)/DHTR plays a crucial role in the development and progression of various cancers, including breast cancer and prostate cancer. Utilizing our highly-validated AR antibody, can significantly enhance the detection and study of AR expression. Research indicates that AR is a promising therapeutic target for treating hormone-dependent cancers, offering potential for improved patient outcomes.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.

Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

Associated diseases and disorders

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

27 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
Image from Azeem W et al., PLoS One. 2017;12(6):e0177861. Fig 6.; doi: 10.1371/journal.pone.0177861. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Texas red (Tx-Red) indirect immunofluorescent detection of Androgen Receptor using ab133273 in (A) EP156T-AR, (B) 957E/hTERT-AR and (C) LNCaP cells. The cells were treated with ± 1 nM R1881 for 24 hours.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Blocking/Diluting buffer: 5% NFDM/TBST
Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602)
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution
Lane 1: Mouse testis lysate at 20 µg
Lane 2: Rat testis lysate at 20 µg
Lane 3: Mouse liver lysate at 20 µg
Lane 4: Rat liver lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
Exposure time: 20s
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution
All lanes: Mouse prostate lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
shao et al Oncotarget. 2018 Mar 6; 9(18): 14456–14471. Published online 2018 Feb 12. doi: 10.18632/oncotarget.24470
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Formalin-fixed, paraffin-embedded tissues labeling Androgen Receptor with ab133273. Left: Tumor from orthotopic injectionof PTEN KD/TE cells. Right: Human postate cancer tissue.
From Figure 3 of Shao et al/
Shao et al Oncotarget. 2018 Mar 6; 9(18): 14456–14471.Published online 2018 Feb 12. doi: 10.18632/oncotarget.24470
Reproduced under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0).
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Androgen receptor with purified ab133273 at 1:100 dilution (1.3μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemical analysis of paraffin-embedded human prostatic adenocarcinoma tissue labelling Androgen Receptor using unpurified ab133273 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Blocking/Diluting buffer - 5% NFDM/TBST
The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.
We recommend you to try both RIPA and 1%SDS Hot lysis preparation methods to get desired bands.
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/20000 dilution
Lane 1: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 1/15 dilution
Lane 2: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 1/15 dilution
Lane 3: 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 1/15 dilution
Lane 4: 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 1/15 dilution
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 120 kDa
Exposure time: 10s
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Different batches of ab133273 were tested on LNCaP (Human prostate carcinoma epithelial cell) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Predicted band size: 99 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunofluorescent staining of LnCAP cells labelling Androgen Receptor using unpurified ab133273, at 1/100 dilution
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution
Lane 1: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: Rat prostate lysates at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 146 kDa, 99 kDa
Observed band size: 160 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution
All lanes: LNCaP (Human prostate carcinoma epithelial cell) at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemical analysis of paraffin-embedded human prostate tissue labelling Androgen Receptor using unpurified ab133273, at1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing positive staining in Endometrial carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Unpurified ab133273 showing positive staining in Prostatic carcinoma T3 tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.