Name: Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade
SKU: ab108341
Rating: 5 (4 reviews)

Anti-Androgen Receptor antibody ER179(2) is a rabbit monoclonal antibody that is used to detect Androgen Receptor in Western blot, IHC, ICC/IF, Flow cytometry, ChIP, IP. Suitable for Human, Mouse, Rat samples.

- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 75 publications
- Antibody clone ER179(2) is cited in over 100 publications

Images

Immunohistochemistry - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (AB108341), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIC/CUT&RUN-seq	IHC-P	IP	ChIP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Not recommended	Tested	Not recommended	Tested	Tested
Mouse	Expected	Tested	Not recommended	Expected	Not recommended	Tested	Expected
Rat	Expected	Expected	Not recommended	Expected	Not recommended	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes For unpurified use at 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer (pH 6.0) or Tris-EDTA buffer (pH9.0) before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes For unpurified use at 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer (pH 6.0) or Tris-EDTA buffer (pH9.0) before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes PubMed: 23817021

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000.00000 - 1/10000.00000	Notes For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Species Rat	Dilution info 1/1000.00000 - 1/10000.00000	Notes For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Species Human	Dilution info 1/1000.00000 - 1/10000.00000	Notes For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes For unpurified use at 1/100 - 1/250.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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15 products for Alternative Product

Target data

See full target information AR

Function

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Alternative names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: ER179(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP, ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) has been cited over 79 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) has 4 independent reviews from customers.

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) specifically detects Androgen Receptor (UniProt ID: P10275; Molecular weight: 99kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone ER179(2) - Anti-Androgen Receptor antibody [ER179(2)] - BSA and Azide free ab212175.

Antibody clone ER179(2) is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594 (PE Anti-CD1a antibody [NA1/34] ab22352, Anti-HLA Class I antibody [W6/32] ab22432, ab2269, ab26358).

The androgen receptor (AR)/DHTR plays a crucial role in the development and progression of various cancers, including breast cancer and prostate cancer. Utilizing our highly-validated AR antibody, can significantly enhance the detection and study of AR expression. Research indicates that AR is a promising therapeutic target for treating hormone-dependent cancers, offering potential for improved patient outcomes. Validated also for CUT&RUN, which is a key applications to map protein-DNA interactions on a genome-wide scale using NGS.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.

Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

Associated diseases and disorders

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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18 product images

Immunohistochemistry - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Immunohistochemical analysis of formalin fixed paraffin embedded human testis labelling androgen receptor with ab108341 at a concentration of 0.19 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.
ab108341 Anti-androgen receptor antibody [ER179(2)] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)
ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Chromatin was prepared from LNCaP cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab108341 (red), or 5 µg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Paper (PMID: 25802280)
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Observed MW; 110 kDa
All lanes: Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution
Lane 1: Mouse testis lysates at 20 µg
Lane 2: Rat testis lysates at 20 µg
Lane 3: Mouse liver lysates at 20 µg
Lane 4: Rat liver lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Exposure time: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Immunohistochemistry (paraffin-embedded sections) analysis of mouse testis tissue labelling Androgen with ab108341 at 0.12 μg/mL for 30mins at room temperature. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/4000). Counterstained with hematoxylin. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Blocking/Diluting buffer: 5% NFDM/TBST
The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.
We recommend you to try both RIPA and 1% SDS Hot lysis preparation methods to get desired bands.
All lanes: Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution
Lane 1: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg
Lane 2: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 15 µg
Lane 3: 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg
Lane 4: 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 120 kDa
Exposure time: 20s
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling Androgen receptor with Purified ab108341 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling Androgen receptor with Purified ab108341 at 1:100 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution
All lanes: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Lanes 1 - 2: Merged signal (red and green). Green - ab108341 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108341 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.
All lanes: Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution
Lane 1: LnCAP whole cell lysate (positive control) at 20 µg
Lane 2: PC3 whole cell lysate (negative control) at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 120 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341, at 1/100, staining Androgen Receptor in LnCaP cells by Immunofluorescence.
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
All lanes: Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution
Lane 1: T47 D cell lysate at 10 µg
Lane 2: LnCaP cell lysate at 10 µg
Predicted band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostatic adenocarcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostate tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341 showing positive staining in Normal testis tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341 showing positive staining in Prostatic carcinoma T3 tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341 showing positive staining in Breast carcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
Unpurified ab108341 showing negative staining in Normal liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ChIC/CUT&RUN sequencing - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 LNCaP (Human prostate carcinoma epithelial cell) cells cultured in phenol red free medium and 5% charcoal stripped FBS for 3 days then treated with DHT (10 nM 4h), and 5 µg of ab108341 [ER179(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.