Rabbit Recombinant Monoclonal Androgen Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38693.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138124.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126366.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Alternative names
DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Recommended products
Rabbit Recombinant Monoclonal Androgen Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP107
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236225 is the carrier-free version of Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
- Biological function summary
AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.
- Pathways
AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.
- Associated diseases and disorders
AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
21 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Androgen Receptor with Purified Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38693.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Androgen Receptor with Purified Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer tissue sections labeling Androgen Receptor with Purified Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138124.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126366.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136765.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137348.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14199.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112558.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human uterus tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human breast tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human testis tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human cervix tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138872.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human placenta tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104096.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139515.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136639.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60924.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97960.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136869.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Androgen Receptor with Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225 at 1/400 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225).
![Western blot - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--western-blot-img431262.jpg "Western blot - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
Western blot - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)
This data was developed using Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (Anti-Androgen Receptor antibody [SP107] - N-terminal ab105225) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
Exposure time: 60s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136765.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137348.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14199.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112558.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135230.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138872.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104096.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139515.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136639.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60924.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97960.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136869.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
![Western blot - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-antibody-sp107-bsa-and-azide-free-ab236225--western-blot-img431262.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (ab236225)")
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